RNA expression profiles and regulatory networks in human right ventricular hypertrophy due to high pressure load.
RNA expression profiles and regulatory networks in human right ventricular hypertrophy due to high pressure load.
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DOI：
10.1016/j.isci.2021.102232
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发表时间：
2021-03-19
期刊：
影响因子：
5.8
通讯作者：
Hansmann G
中科院分区：
文献类型：
--
作者：
Chouvarine P;Photiadis J;Cesnjevar R;Scheewe J;Bauer UMM;Pickardt T;Kramer HH;Dittrich S;Berger F;Hansmann G
右心室肥大（RVH）发生在高压后负荷，例如，法洛四联症/肺动脉狭窄（TOF/PS）。这种RVH与能量代谢、神经激素和表观遗传失调（例如，microRNA）和动物模型中的胎儿基因重编程。然而，尚未对人RVH中的竞争性内源性RNA进行全面的表达谱分析。在这里，我们解开了几个以前未知的环状，长非编码，和microRNA，预测调控基因的表达，人类RVH在非衰竭心脏（TOF/PS）。这些基因在与调节葡萄糖和脂质代谢（SIK 1、FABP 4）、细胞表面相互作用（THBS 2、FN 1）、细胞凋亡（PIK 3 IP 1、SIK 1）、细胞外基质组成（CTGF、IGF 1）和其他生物学事件相关的途径中显著过量表达。这是人类代偿性RVH的第一个无偏RNA测序研究，包括编码和非编码RNA表达，并预测了非编码RNA对miRNA的海绵作用。这些发现推进了我们对适应性RVH的理解，并突出了未来的治疗目标。通过RNA表达和网络分析首次对人类RVH进行全面的转录组学研究首次使用新鲜分离的心肌进行人类RVH研究已知肥大基因受到RVH中竞争性内源性RNA网络的最强调控RVH中ncRNA的表观遗传mRNA调控依赖于性别和年龄分子生物学;组学
Right ventricular hypertrophy (RVH) occurs in high pressure afterload, e.g., tetralogy of Fallot/pulmonary stenosis (TOF/PS). Such RVH is associated with alterations in energy metabolism, neurohormonal and epigenetic dysregulation (e.g., microRNA), and fetal gene reprogramming in animal models. However, comprehensive expression profiling of competing endogenous RNA in human RVH has not been performed. Here, we unravel several previously unknown circular, long non-coding, and microRNAs, predicted to regulate expression of genes specific to human RVH in the non-failing heart (TOF/PS). These genes are significantly overrepresented in pathways related to regulation of glucose and lipid metabolism (SIK1, FABP4), cell surface interactions (THBS2, FN1), apoptosis (PIK3IP1, SIK1), extracellular matrix composition (CTGF, IGF1), and other biological events. This is the first unbiased RNA sequencing study of human compensated RVH encompassing coding and non-coding RNA expression and predicted sponging of miRNAs by non-coding RNAs. These findings advance our understanding of adaptive RVH and highlight future therapeutic targets. First comprehensive transcriptomic study of human RVH via RNA expression and network analysis First human RVH study using exclusively freshly isolated myocardium Known hypertrophy genes are regulated the strongest by competing endogenous RNA networks in RVH Epigenetic mRNA regulation in RVH by ncRNAs is dependent on sex and age Molecular Biology; Omics
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影响因子：
20.1
作者：
Israeli-Rosenberg S;Manso AM;Okada H;Ross RS
通讯作者：
Ross RS
影响因子：
6
作者：
Jakobi, Tobias;Siede, Dominik;Dieterich, Christoph
通讯作者：
Dieterich, Christoph
影响因子：
4.7
作者：
Chouvarine, Philippe;Legchenko, Ekaterina;Hansmann, Georg
通讯作者：
Hansmann, Georg
影响因子：
37.8
作者：
Andersen, Stine;Nielsen-Kudsk, Jens Erik;de Man, Frances S.
通讯作者：
de Man, Frances S.
DOI：
10.1165/rcmb.2013-0149oc
发表时间：
2013-12-01
影响因子：
6.4
作者：
Friedberg, Mark K.;Cho, Mi-Young;Redington, Andrew N.
通讯作者：
Redington, Andrew N.