RecQ Helicases Function in Development, DNA Repair, and Gene Targeting in Physcomitrella patens[OPEN]
RecQ Helicases Function in Development, DNA Repair, and Gene Targeting in Physcomitrella patens[OPEN]
复制标题
RecQ 解旋酶在小立碗藓发育、DNA 修复和基因靶向中的功能[打开]
DOI：
--
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
R. Reski
中科院分区：
文献类型：
--
作者：
Gertrud Wiedemann;Nico van Gessel;Fabian Köchl;Lisa Hunn;Katrin Schulze;Lina Maloukh;F. Nogué;E. Decker;F. Hartung;R. Reski
RecQ DNA解旋酶是在分生组织中发现的重要的基因组监视蛋白，在植物发育、DNA修复和基因靶向中起作用。RecQ DNA解旋酶是存在于所有生命体中的基因组监视蛋白。它们在人类中表现最好，因为RecQ基因的突变会导致发育异常和疾病。为了更好地了解RecQ在植物中的功能，我们重点研究了拟南芥和小立小藻这两种主要用于DNA修复和基因靶向研究的模式物种。6个基因的系统发育分析揭示了它们在人类和植物中的同源性。由于拟南芥和P. patens的RecQ4和RecQ6基因不同，因此产生了报告和缺失苔藓突变体，并通过互惠的跨物种和跨界方法研究了基因功能。这两种蛋白都可以在分生苔藓组织中发现，尽管水平较低且表达模式不同。PpRecQ4参与胚胎发生和随后的发育，如ΔPpRecQ4突变体的不育和形态畸变所证明的那样。此外，ΔPpRecQ4对DNA损伤的敏感性增加，基因靶向率增加。因此，我们得出结论，PpRecQ4作为重组的抑制因子。相比之下，PpRecQ6对苔藓发育或DNA修复并不明显重要，但确实是基因靶向的有效增强子。
RecQ DNA helicases are important genome surveillance proteins that are found in meristematic tissues, where they function in plant development, DNA repair, and gene targeting in the moss Physcomitrella patens. RecQ DNA helicases are genome surveillance proteins found in all kingdoms of life. They are characterized best in humans, as mutations in RecQ genes lead to developmental abnormalities and diseases. To better understand RecQ functions in plants we concentrated on Arabidopsis thaliana and Physcomitrella patens, the model species predominantly used for studies on DNA repair and gene targeting. Phylogenetic analysis of the six P. patens RecQ genes revealed their orthologs in humans and plants. Because Arabidopsis and P. patens differ in their RecQ4 and RecQ6 genes, reporter and deletion moss mutants were generated and gene functions studied in reciprocal cross-species and cross-kingdom approaches. Both proteins can be found in meristematic moss tissues, although at low levels and with distinct expression patterns. PpRecQ4 is involved in embryogenesis and in subsequent development as demonstrated by sterility of ΔPpRecQ4 mutants and by morphological aberrations. Additionally, ΔPpRecQ4 displays an increased sensitivity to DNA damages and an increased rate of gene targeting. Therefore, we conclude that PpRecQ4 acts as a repressor of recombination. In contrast, PpRecQ6 is not obviously important for moss development or DNA repair but does function as a potent enhancer of gene targeting.
登录
查看更多内容
影响因子：
7.2
作者：
Lang, Daniel;Ullrich, Kristian K.;Rensing, Stefan A.
通讯作者：
Rensing, Stefan A.
影响因子：
56.9
作者：
Rensing, Stefan A.;Lang, Daniel;Boore, Jeffrey L.
通讯作者：
Boore, Jeffrey L.
影响因子：
7.2
作者：
Higgins, James D.;Ferdous, Maheen;Franklin, F. Christopher H.
通讯作者：
Franklin, F. Christopher H.
影响因子：
16.6
作者：
Croteau DL;Popuri V;Opresko PL;Bohr VA
通讯作者：
Bohr VA
影响因子：
4.5
作者：
Röhrig S;Schröpfer S;Knoll A;Puchta H
通讯作者：
Puchta H