Utilizing virus-induced gene silencing for the functional characterization of maize genes during infection with the fungal pathogen Ustilago maydis.
Utilizing virus-induced gene silencing for the functional characterization of maize genes during infection with the fungal pathogen Ustilago maydis.
复制标题
利用病毒诱导的基因沉默来表征真菌病原体玉米黑粉菌感染期间玉米基因的功能特征
DOI：
10.1007/978-1-62703-278-0_4
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发表时间：
2012
影响因子：
--
通讯作者：
Doehlemann G
中科院分区：
文献类型：
--
作者：
van der Linde K;Mueller A;Hemetsberger C;Kaschani F;van der Hoorn RAL;Doehlemann G
虽然在双子叶植物中，病毒诱导的基因沉默(VIGS)已经很好地被用来研究植物与病原菌的相互作用，但到目前为止，在单子叶植物中有效的VIGS还没有报道。其中一个有效的系统是基于雀麦花叶病毒(BMV)，它允许大麦(Hordeum Visgare)、小麦(Triticum Aestivum)和玉米(Zea Mays)等不同谷物的基因沉默。玉米黑穗病Ustilago感染玉米植株可能导致茎、叶和花序上形成大肿瘤。在这种生物营养相互作用中，植物的防御反应被病原菌主动抑制，先前对感染玉米植株的转录组分析显示，在疾病进展过程中寄主基因表达发生了全面的和阶段特异性的变化。为了鉴定与玉米大麦互作相关的功能基因，我们在允许成功消毒预感染玉米的条件下，将基于雀麦花叶病毒(BMV)的VIGS系统改造到玉米上。这一设置使得能够使用基于定量实时PCR(Q(RT)-PCR)的读数来量化VIGS及其对U的影响。
While in dicotyledonous plants virus-induced gene silencing (VIGS) is well established to study plant–pathogen interaction, in monocots only few examples of efficient VIGS have been reported so far. One of the available systems is based on the brome mosaic virus (BMV) which allows gene silencing in different cereals including barley (Hordeum vulgare), wheat (Triticum aestivum), and maize (Zea mays).Infection of maize plants by the corn smut fungusUstilago maydisleads to the formation of large tumors on stem, leaves, and inflorescences. During this biotrophic interaction, plant defense responses are actively suppressed by the pathogen, and previous transcriptome analyses of infected maize plants showed comprehensive and stage-specific changes in host gene expression during disease progression.To identify maize genes that are functionally involved in the interaction withU.maydis, we adapted a VIGS system based on theBrome mosaic virus(BMV) to maize at conditions that allow successfulU.maydisinfection of BMV pre-infected maize plants. This setup enables quantification of VIGS and its impact onU.maydisinfection using a quantitative real-time PCR (q(RT)-PCR)-based readout.
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影响因子：
9.4
作者：
van der Linde, Karina;Kastner, Christine;Doehlemann, Gunther
通讯作者：
Doehlemann, Gunther
DOI：
10.1073/pnas.0711406105
发表时间：
2008-02
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
A. Sindhu;Satya Chintamanani;Amanda S. Brandt;M. Zanis;S. Scofield;G. Johal
通讯作者：
A. Sindhu;Satya Chintamanani;Amanda S. Brandt;M. Zanis;S. Scofield;G. Johal
影响因子：
7.2
作者：
Doehlemann, Gunther;Wahl, Ramon;Kaemper, Joerg
通讯作者：
Kaemper, Joerg
影响因子：
56.9
作者：
Skibbe, David S.;Doehlemann, Gunther;Walbot, Virginia
通讯作者：
Walbot, Virginia