The cloning and characterization of two ammonium transporters in the salt-resistant green alga, Dunaliella viridis
The cloning and characterization of two ammonium transporters in the salt-resistant green alga, Dunaliella viridis
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耐盐绿藻绿色杜氏藻中两种铵转运蛋白的克隆和表征
DOI：
10.1007/s11033-010-0621-1
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Rentao Song
中科院分区：
文献类型：
--
作者：
Ting Song;Qiang Gao;Zhengkai Xu;Rentao Song
铵离子（NH 4（+））转运是氮代谢的关键过程。为了研究盐胁迫下盐生杜氏藻（Dunaliella viridis）的铵转运蛋白在盐胁迫下盐生杜氏藻氮消耗中的作用，从杜氏藻cDNA文库中克隆了两个铵转运蛋白基因DvAMT 1;1和DvAMT 1;2。绿色的DvAMT 1;1和DvAMT 1;2只有40%的氨基酸相同性，表明它们具有高度不同的编码序列。功能互补的酵母突变体缺陷的铵吸收表明，DvAMT 1;1和DvAMT 1;2功能铵转运蛋白。定量RT-PCR显示相似的表达模式，但不同的转录丰度水平，DvAMT 1;1和DvAMT 1;2在不同的氮条件下。二者在低氮条件下均被诱导，在高氮条件下被抑制，尤其是以NH（4）（+）为氮源时。在转录水平上，DvAMT 1;1受昼夜调控，而DvAMT 1;2不受昼夜调控。此外，在0.5至3 M的NaCl浓度范围内，DvAMT 1;1在高盐条件下下调;相反，DvAMT 1;2保持相对较低但稳定的转录丰度。观察到的DvAMT 1;1和DvAMT 1;2在转录调控上的差异表明它们在D.绿色的
Ammonium (NH(4) (+)) transport is a key process in nitrogen metabolism. To elucidate the role of ammonium transporters in the nitrogen consumption of the salt-resistant green alga, Dunaliella viridis, two ammonium transporter genes, DvAMT1;1 and DvAMT1;2, were isolated from cDNA libraries of D. viridis. DvAMT1;1 and DvAMT1;2 share only 40% amino acid identity, indicating that they have highly divergent coding sequences. Functional complementation in a yeast mutant defective in ammonium uptake indicated that both DvAMT1;1 and DvAMT1;2 were functional ammonium transporters. Quantitative RT-PCR showed similar expression patterns, but different transcript abundance levels, for DvAMT1;1 and DvAMT1;2 under different nitrogen conditions. Both were induced at low nitrogen and inhibited at high nitrogen concentrations, especially when NH(4) (+) was the nitrogen source. At the transcriptional level, DvAMT1;1 was diurnally regulated, while DvAMT1;2 was not. In addition, under NaCl concentrations that ranged from 0.5 to 3 M, DvAMT1;1 was down-regulated at the higher salt conditions; conversely, DvAMT1;2 maintained a relatively low, but stable, transcript abundance. The observed differences in transcriptional regulation of DvAMT1;1 and DvAMT1;2 are indicative of their diverse physiological functions in D. viridis.
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DOI：
10.1083/jcb.115.4.887
发表时间：
1991-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kozak M
通讯作者：
Kozak M
影响因子：
5.1
作者：
J. N. Pearson;J. Finnemann;J. Schjoerring
通讯作者：
J. N. Pearson;J. Finnemann;J. Schjoerring
影响因子：
7.4
作者：
Liska, AJ;Shevchenko, A;Katz, A
通讯作者：
Katz, A
影响因子：
4.9
作者：
Sonoda, Y;Ikeda, A;Yamaguchi, J
通讯作者：
Yamaguchi, J
影响因子：
6.9
作者：
LEE, RB;PURVES, JV;SAKER, LR
通讯作者：
SAKER, LR