Gold nanoparticles conjugated with DNA aptamer for photoacoustic detection of human matrix metalloproteinase-9.
Gold nanoparticles conjugated with DNA aptamer for photoacoustic detection of human matrix metalloproteinase-9.
复制标题
金纳米粒子与DNA适配子的偶联光声检测人基质金属蛋白酶-9。
DOI：
10.1016/j.pacs.2021.100307
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发表时间：
2022-03
期刊：
影响因子：
7.9
通讯作者：
Emelianov SY
中科院分区：
文献类型：
--
作者：
Kim J;Yu AM;Kubelick KP;Emelianov SY
Matrix metalloproteinase-9 (MMP-9) plays major roles in extracellular matrix (ECM) remodeling and membrane protein cleavage, suggesting a high correlation with cancer cell invasion and tumor metastasis. Here, we present a contrast agent based on a DNA aptamer that can selectively target human MMP-9 in the tumor microenvironment (TME) with high affinity and sensitivity. Surface modification of plasmonic gold nanospheres with the MMP-9 aptamer and its complementary sequences allows the nanospheres to aggregate in the presence of human MMP-9 through DNA displacement and hybridization. Aggregation of gold nanospheres enhances the optical absorption in the first near-infrared window (NIR-I) due to the plasmon coupling effect, thereby allowing us to detect the aggregated gold nanospheres within the TME via ultrasound-guided photoacoustic (US/PA) imaging. Selective and sensitive detection of human MMP-9 via US/PA imaging is demonstrated in solution of nanosensors with the pre-treatment of human MMP-9, in vitro in cell culture, and in vivo in a xenograft murine model of human breast cancer.
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用于光声成像的金纳米粒子
DOI：
10.2217/nnm.14.169
发表时间：
2015-01
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Li W;Chen X
通讯作者：
Chen X
影响因子：
6.8
作者：
Cathcart, Jillian;Pulkoski-Gross, Ashleigh;Cao, Jian
通讯作者：
Cao, Jian
影响因子：
14
作者：
Lee, Hyori;Kim, Jinhwan;Kim, Won Jong
通讯作者：
Kim, Won Jong
影响因子：
29.4
作者：
Kim, Jinhwan;Jo, Changshin;Kim, Won Jong
通讯作者：
Kim, Won Jong
影响因子：
17.1
作者：
Kim, Jinhwan;Lee, Yeong Mi;Kim, Won Jong
通讯作者：
Kim, Won Jong