The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid.
The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid.
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DOI：
10.1186/s12862-020-01733-1
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发表时间：
2021-01-25
影响因子：
2.2
通讯作者：
Nozaki H
中科院分区：
文献类型：
--
作者：
Matsuzaki R;Suzuki S;Yamaguchi H;Kawachi M;Kanesaki Y;Yoshikawa H;Mori T;Nozaki H
淀粉核是主要由核酮糖 - 1,5 - 二磷酸羧化酶/加氧酶（Rubisco）组成的蛋白质微区室，位于许多光合生物的叶绿体中。淀粉核有助于二氧化碳浓缩机制。在藻类/植物进化过程中，这种细胞器多次丢失，包括在陆地植物起源时。淀粉核进化丢失的分子基础是进化生物学的一个重要课题。最近，有人假设淀粉核的形成受Rubisco小亚基（RBCS）表面两个螺旋的疏水性控制，但在密切相关的藻类/植物谱系进化过程中，疏水性与淀粉核丢失之间的关系尚未得到研究。在此，我们关注单细胞绿藻属衣藻（Chloromonas）的网纹组（Reticulata group），其中一些物种存在淀粉核，而在密切相关的物种中则不存在。
基于从头转录组分析以及对克隆的逆转录 - 聚合酶链反应产物进行桑格测序，我们从网纹组两个无淀粉核物种和三个有淀粉核物种的11个菌株中确定了rbcS序列。我们发现，在网纹组内，RBCS螺旋的疏水性与淀粉核的有无大致相关，并且RBCS螺旋疏水性的降低可能是该组进化过程中淀粉核丢失的主要原因。
尽管我们认为所观察到的相关性可能仅存在于网纹组，但这仍然是一项有趣的研究，为确定淀粉核获得和丢失的初始进化步骤的潜在机制提供了新的见解。
Pyrenoids are protein microcompartments composed mainly of Rubisco that are localized in the chloroplasts of many photosynthetic organisms. Pyrenoids contribute to the CO2-concentrating mechanism. This organelle has been lost many times during algal/plant evolution, including with the origin of land plants. The molecular basis of the evolutionary loss of pyrenoids is a major topic in evolutionary biology. Recently, it was hypothesized that pyrenoid formation is controlled by the hydrophobicity of the two helices on the surface of the Rubisco small subunit (RBCS), but the relationship between hydrophobicity and pyrenoid loss during the evolution of closely related algal/plant lineages has not been examined. Here, we focused on, the Reticulata group of the unicellular green algal genus Chloromonas, within which pyrenoids are present in some species, although they are absent in the closely related species. Based on de novo transcriptome analysis and Sanger sequencing of cloned reverse transcription-polymerase chain reaction products, rbcS sequences were determined from 11 strains of two pyrenoid-lacking and three pyrenoid-containing species of the Reticulata group. We found that the hydrophobicity of the RBCS helices was roughly correlated with the presence or absence of pyrenoids within the Reticulata group and that a decrease in the hydrophobicity of the RBCS helices may have primarily caused pyrenoid loss during the evolution of this group. Although we suggest that the observed correlation may only exist for the Reticulata group, this is still an interesting study that provides novel insight into a potential mechanism determining initial evolutionary steps of gain and loss of the pyrenoid.
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