Red blood cell microparticles show altered inflammatory chemokine binding and release ligand upon interaction with platelets.
Red blood cell microparticles show altered inflammatory chemokine binding and release ligand upon interaction with platelets.
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红细胞微粒显示出改变的炎症趋化因子结合并在与血小板相互作用时释放配体。
DOI：
10.1111/j.1537-2995.2010.02861.x
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发表时间：
2011-03
期刊：
影响因子：
2.9
通讯作者：
Lee JS
中科院分区：
文献类型：
--
作者：
Xiong Z;Cavaretta J;Qu L;Stolz DB;Triulzi D;Lee JS
在标准血库条件下储存红细胞(RBC)会导致结构完整性降低，导致膜萌发和微粒释放。微粒子表达已知能结合多种炎性趋化因子的Duffy血型抗原，但微粒子的功能性趋化因子结合特性尚不清楚。我们通过Duffy抗原的表达来确定储存诱导的微粒子是否显示了炎性趋化因子结合，比较了与完整红细胞的结合特性，并评估了微粒子与激活时释放趋化因子的血小板(PLT)的相互作用。在整个保存过程中，完整的红细胞对CCl2(Kd=7.4±0.9nmol/L)、CXCl8(Kd=7.9±1.0nmol/L)和CXCl1(Kd=4.4±1.0nmo1/L)保持着相似的平衡解离常数。相反，随着储存时间的增加，微粒的相对计数增加，表面磷脂酰丝氨酸的百分比增加，并表现出降低的Duffy依赖的趋化因子结合亲和力，与CXCL1的解离常数变化较大(Kd=362±328nmol/L；范围为0.6-2000nmol/L)。RBC微粒趋化因子结合亲和力的改变与与PLT孵育时释放配体的倾向有关。基于血糖素A表达和大小标准的微粒相对定量低估了与功能性趋化因子结合的颗粒数量，表明血糖素A阴性颗粒和纳米粒有助于总的趋化因子结合能力。由功能性趋化因子结合决定的输液中的微粒负荷是相当大的。红细胞微粒膜性质的改变增强了PLT的相互作用，从而提高了炎性趋化因子的体外生物利用度。
Storage of red blood cells (RBCs) under standard blood bank conditions results in reduced structural integrity leading to membrane budding and release of microparticles. Microparticles express the blood group Duffy antigen known to bind multiple inflammatory chemokines, but the functional chemokine binding properties of microparticles are not known. We determined whether storage-induced microparticles show inflammatory chemokine binding through the expression of the Duffy antigen, comparing the binding properties to intact RBCs, and assessed microparticle interactions with platelets (PLTs) that release chemokines upon activation. Intact RBCs retained similar equilibrium dissociation constants for CCL2 (Kd = 7.4 ± 0.9 nmol/L), CXCL8 (Kd = 7.9 ± 1.0 nmol/L), and CXCL1 (Kd = 4.4 ± 1.0 nmol/L) throughout storage. In contrast, microparticles increased in relative counts with storage, showed higher percentages of surface phosphatidylserine, and demonstrated impaired Duffy-dependent chemokine binding affinity with wider variability in dissociation constant for CXCL1(Kd = 362 ± 328 nmol/L; range, 0.6–2000 nmol/L). The altered chemokine binding affinity of RBC microparticles was associated with a propensity to release ligand upon incubation with PLTs. Relative quantification of microparticles, based on criteria of glycophorin A expression and size, underestimated particle numbers with functional chemokine binding, suggesting that glycophorin A–negative particles and nanoparticles contribute to overall chemokine binding capacity. Microparticle burden in transfusates, as determined by functional chemokine binding, is considerable. Altered membrane properties of RBC microparticles enhance PLT interactions to increase inflammatory chemokine bioavailability in vitro.
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影响因子：
20.3
作者：
Schnabel, Renate B.;Baumert, Jens;Tracy, Russell P.
通讯作者：
Tracy, Russell P.
影响因子：
20.3
作者：
Mangalmurti, Nilam S.;Xiong, Zeyu;Lee, Janet S.
通讯作者：
Lee, Janet S.
影响因子：
2.9
作者：
An, XL;Guo, XH;Mohandas, N
通讯作者：
Mohandas, N
DOI：
10.1161/01.atv.0000170133.43608.37
发表时间：
2005-07-01
影响因子：
8.7
作者：
Mause, SF;von Hundelshausen, P;Weber, C
通讯作者：
Weber, C
影响因子：
158.5
作者：
MILLER, LH;MASON, SJ;MCGINNISS, MH
通讯作者：
MCGINNISS, MH