Molecular identification of Coccidioides spp. in soil samples from Brazil.
Molecular identification of Coccidioides spp. in soil samples from Brazil.
复制标题
DOI：
10.1186/1471-2180-11-108
复制
发表时间：
2011-05-16
期刊：
影响因子：
4.2
通讯作者：
Wanke B
中科院分区：
文献类型：
--
作者：
de Macêdo RC;Rosado AS;da Mota FF;Cavalcante MA;Eulálio KD;Filho AD;Martins LM;Lazéra MS;Wanke B
自1991年以来，在巴西东北部半干旱地区发现了几起急性球孢子菌病(CM)暴发，主要与狩猎者为捕获这些动物而挖掘它们时对它们的洞穴造成的干扰有关。这种活动会导致粉尘被波萨德球孢子虫的节孢子污染，一旦吸入，就会导致真菌病。我们报告了在与CM暴发有关的土壤样本中鉴定Posadasii的情况。24个土壤样本的DNA被提取出来，然后用特定的引物提交给半套式PCR技术。小鼠接种只有6个土样(25%)阳性，而分子工具检测全部阳性(100%)。该方法是一种简单、灵敏、特异的确定球孢子虫环境分布的分子技术。在流行地区，但不能区分物种。此外，它可能有助于鉴定培养分离株。关键词：1.球孢子菌病。2.球球藻Coccidioidesspp.3.C.posadasii。4.半干旱。5.半套式聚合酶链式反应
Since 1991 several outbreaks of acute coccidioidomycosis (CM) were diagnosed in the semi-arid Northeast of Brazil, mainly related to disturbance of armadillo burrows caused by hunters while digging them for the capture of these animals. This activity causes dust contaminated with arthroconidia of Coccidioides posadasii, which, once inhaled, cause the mycosis. We report on the identification of C. posadasii in soil samples related to outbreaks of CM. Twenty four soil samples had their DNA extracted and subsequently submitted to a semi-nested PCR technique using specific primers. While only 6 (25%) soil samples were positive for C. posadasii by mice inoculation, all (100%) were positive by the molecular tool. This methodology represents a simple, sensitive and specific molecular technique to determine the environmental distribution of Coccidioides spp. in endemic areas, but cannot distinguish the species. Moreover, it may be useful to identify culture isolates. Key-words: 1. Coccidioidomycosis. 2. Coccidioides spp. 3. C. posadasii. 4. Semi-arid. 5. Semi-nested PCR
登录
查看更多内容
影响因子：
15.8
作者：
Hector RF;Laniado-Laborin R
通讯作者：
Laniado-Laborin R
影响因子：
3.3
作者：
Emmons, C. W.
通讯作者：
Emmons, C. W.
影响因子：
13.8
作者：
Jeanmougin, F;Thompson, JD;Gibson, TJ
通讯作者：
Gibson, TJ
影响因子：
5.5
作者：
Eulálio, KD;de Macedo, RL;Wanke, B
通讯作者：
Wanke, B
影响因子：
3.2
作者：
ELCONIN, AF;EGEBERG, RO;EGEBERG, MC
通讯作者：
EGEBERG, MC