Circulating microRNAs: a novel potential biomarker for diagnosing acute aortic dissection.
Circulating microRNAs: a novel potential biomarker for diagnosing acute aortic dissection.
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循环 microRNA：诊断急性主动脉夹层的新型潜在生物标志物
DOI：
10.1038/s41598-017-13104-w
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发表时间：
2017-10-06
影响因子：
4.6
通讯作者：
Jing Z
中科院分区：
文献类型：
--
作者：
Dong J;Bao J;Feng R;Zhao Z;Lu Q;Wang G;Li H;Su D;Zhou J;Jing Q;Jing Z
急性主动脉夹层是一种灾难性急症，死亡率高，误诊率高。我们的目的是确定循环microRNA是否允许区分AAD与健康对照和无AAD的胸痛患者（CP）。检测103例受试者血浆microRNA的表达，其中AAD患者37例，慢性主动脉夹层患者26例，健康志愿者17例，非AAD患者23例。我们从微阵列筛选中选择了16种microRNA作为通过qRT-PCR进行进一步测试的候选物。结果显示，AAD患者（n = 37）血浆miR-15 a的表达水平显著高于对照组（n = 40;P= 0.008）。经受试者工作特征曲线分析，AAD检测的敏感性为75.7%，特异性为82.5%，AUC为0.761。此外，37例AAD患者的let-7 b、miR-15 a、miR-23 a和hcmv-miR-US 33 - 5 p的血浆表达水平显著高于40例对照组中的14例CP患者（P分别为0.000、0.000、0.026和0.011）。其敏感性分别为79.4%、75.7%、91.9%和73.5%，特异性分别为92.9%、100%、85.7%和85.7%，AUC分别为0.887、0.855、0.925和0.815。这些数据表明，血浆miR-15 a和miR-23 a在诊断AAD中具有很好的临床价值。
Acute aortic dissection (AAD) is a catastrophic emergency with high mortality and misdiagnosis rate. We aimed to determine whether circulating microRNAs allow to distinguish AAD from healthy controls and chest pain patients without AAD (CP). Plasma microRNAs expression were determined in 103 participants, including 37 AAD patients, 26 chronic aortic dissection patients, 17 healthy volunteers, 23 patients without AAD. We selected 16 microRNAs from microarray screening as candidates for further testing via qRT-PCR. The results showed that plasma miR-15a in patients with AAD (n = 37) had significantly higher expression levels than it from control group (n = 40;P= 0.008). By receiver operating characteristic curve analysis, the sensitivity was 75.7%; the specificity was 82.5%; and the AUC was 0.761 for detection of AAD. Furthermore, 37 patients with AAD had significantly higher plasma expression levels of let-7b, miR-15a, miR-23a and hcmv-miR-US33-5p compared with 14 CP patients of 40 controls (P= 0.000, 0.000, 0.026 and 0.011, respectively). The corresponding sensitivity were 79.4%, 75.7%, 91.9% and 73.5%, respectively; the specificity were 92.9%, 100%, 85.7% and 85.7%, respectively; and the AUCs of these microRNAs were 0.887, 0.855, 0.925 and 0.815, respectively. These data indicate that plasma miR-15a and miR-23a have promising clinical value in diagnosing AAD.
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影响因子：
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