MiR-34a, miR-21 and miR-23a as potential biomarkers for coronary artery disease: a pilot microarray study and confirmation in a 32 patient cohort.
MiR-34a, miR-21 and miR-23a as potential biomarkers for coronary artery disease: a pilot microarray study and confirmation in a 32 patient cohort.
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DOI：
10.1038/emm.2014.81
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发表时间：
2015-02-06
影响因子：
12.8
通讯作者：
Luo, Shanshun
中科院分区：
文献类型：
--
作者：
Han, Hui;Qu, Guangjin;Han, Chenghua;Wang, Yuhong;Sun, Tingting;Li, Fengqing;Wang, Junxiao;Luo, Shanshun
本研究的目的是研究载脂蛋白 E (apoE) 敲除小鼠 (apoE−/−) 中循环 microRNA (miRNA) 的表达，并验证这些 miRNA 在人类冠状动脉疾病 (CAD) 中的作用。使用来自 10 只 apoE−/− 小鼠和 10 只健康 C57BL/6 (B6) 小鼠的混合血浆进行微阵列分析。结果显示，miR-34a、miR-21、miR-23a、miR-30a和miR-106b在apoE−/−小鼠中存在差异表达，并且这些表达变化通过实时定量逆转录PCR得到证实。然后，在 32 名 CAD 患者和 20 名健康对照者的血浆中检测到 miR-34a、miR-21、miR-23a、miR-30a 和 miR-106b。 CAD患者血浆中仅miR-34a、miR-21和miR-23a表达存在显着差异（均P<0.01）。总之，miR-34a、miR-21 和 miR-23a 在 CAD 患者中升高，这意味着这些 miRNA 可能作为 CAD 发生和进展的生物标志物。
The aim of this study was to investigate the expression of circulating microRNAs (miRNAs) in apolipoprotein E (apoE) knockout mice (apoE−/−) and to validate the role of these miRNAs in human coronary artery disease (CAD). Pooled plasma from 10 apoE−/− mice and 10 healthy C57BL/6 (B6) mice was used to perform the microarray analysis. The results showed that miR-34a, miR-21, miR-23a, miR-30a and miR-106b were differentially expressed in apoE−/− mice, and these expression changes were confirmed by real-time quantitative reverse-transcription PCR. Then, miR-34a, miR-21, miR-23a, miR-30a and miR-106b were detected in the plasma of 32 patients with CAD and of 20 healthy controls. Only miR-34a, miR-21 and miR-23a were significantly differentially expressed in the plasma of CAD patients (all P<0.01). In conclusion, miR-34a, miR-21 and miR-23a were elevated in CAD patients, which means that these miRNAs might serve as biomarkers of CAD development and progression.
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影响因子：
64.8
作者：
He, Lin;He, Xingyue;Hannon, Gregory J.
通讯作者：
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影响因子：
18.2
作者：
Dickinson, Brent A.;Semus, Hillary M.;van Rooij, Eva
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Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
3.7
作者：
Gilad S;Meiri E;Yogev Y;Benjamin S;Lebanony D;Yerushalmi N;Benjamin H;Kushnir M;Cholakh H;Melamed N;Bentwich Z;Hod M;Goren Y;Chajut A
通讯作者：
Chajut A
DOI：
10.1161/01.atv.0000036081.01231.16
发表时间：
2002-11-01
影响因子：
8.7
作者：
Bea, F;Blessing, E;Rosenfeld, ME
通讯作者：
Rosenfeld, ME