Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos.
Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos.
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DOI：
10.1016/j.taap.2012.08.007
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发表时间：
2012-10-15
影响因子：
3.8
通讯作者：
Hahn, Mark E.
中科院分区：
文献类型：
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作者：
Jenny, Matthew J.;Aluru, Neelakanteswar;Hahn, Mark E.
虽然许多药物和环境化学品是致畸的，但大多数毒物扰乱胚胎发育的机制尚不清楚。MicroRNAs是一种~22nT的单链RNA分子，通过抑制mRNA的翻译和促进mRNA的隔离或降解来调节蛋白质的表达，是包括胚胎发育和细胞分化在内的各种细胞过程的重要调节因子。最近的研究表明，接触外源生物可以改变microRNA的表达，并有助于环境化学品扰乱胚胎发育的机制。在这项研究中，我们检验了发育过程中暴露于2，3，7，8-四氯二苯并对二恶英(TCDD)的假设，TCDD是一种众所周知的致畸物质，在斑马鱼发育过程中改变了microRNA的表达。我们在受精后30h将斑马鱼胚胎暴露于DMSO(0.1%)或TCDD(5 NM)中1小时，并在36和60HPF时用几种方法测定了microRNA的表达。TCDD在36HPF(62倍)和60HPF(135倍)处引起强烈的CYP1A诱导，证实了暴露的有效性。使用微阵列(Agilent和Exiqon)、下一代测序(Solid)和实时RT-PCR来确定microRNA的表达谱。两个微阵列平台的结果相似但不完全相同；在60hpf时，两者都显示miR-451、23a、23b、24和27e的表达发生了显著变化。对TCDD在斑马鱼胚胎中潜在差异表达的16个microRNAs的固体序列进行了多次分析。然而，miR-27e是唯一被鉴定为在所有三种方法(微阵列、固体测序和实时RT-PCR)中差异表达的microRNA。这些结果表明，接触TCDD会引起microRNAs表达的轻微变化，包括一些对造血和心血管发育至关重要的microRNAs(miR-451、23a、23b、24和27e)。
Although many drugs and environmental chemicals are teratogenic, the mechanisms by which most toxicants disrupt embryonic development are not well understood. MicroRNAs, single-stranded RNA molecules of ~22 nt that regulate protein expression by inhibiting mRNA translation and promoting mRNA sequestration or degradation, are important regulators of a variety of cellular processes including embryonic development and cellular differentiation. Recent studies have demonstrated that exposure to xenobiotics can alter microRNA expression and contribute to the mechanisms by which environmental chemicals disrupt embryonic development. In this study we tested the hypothesis that developmental exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a well-known teratogen, alters microRNA expression during zebrafish development. We exposed zebrafish embryos to DMSO (0.1%) or TCDD (5 nM) for 1 hr at 30 hours post fertilization (hpf) and measured microRNA expression using several methods at 36 and 60 hpf. TCDD caused strong induction of CYP1A at 36 hpf (62-fold) and 60 hpf (135-fold) as determined by real-time RT-PCR, verifying the effectiveness of the exposure. MicroRNA expression profiles were determined using microarrays (Agilent and Exiqon), next-generation sequencing (SOLiD), and real-time RT-PCR. The two microarray platforms yielded results that were similar but not identical; both showed significant changes in expression of miR-451, 23a, 23b, 24 and 27e at 60 hpf. Multiple analyses were performed on the SOLiD sequences yielding a total of 16 microRNAs as potentially differentially expressed by TCDD in zebrafish embryos. However, miR-27e was the only microRNA to be identified as differentially expressed by all three methods (both microarrays, SOLiD sequencing, and real-time RT-PCR). These results suggest that TCDD exposure causes modest changes in expression of microRNAs, including some (miR-451, 23a, 23b, 24 and 27e) that are critical for hematopoiesis and cardiovascular development.
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影响因子：
37.3
作者：
Chhabra R;Dubey R;Saini N
通讯作者：
Saini N
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
测量 microRNA：微阵列和定量 PCR 测量以及不同总 RNA 制备方法的比较。
影响因子：
3.5
作者：
Ach, Robert A.;Wang, Hui;Curry, Bo
通讯作者：
Curry, Bo
影响因子：
2.8
作者：
Halappanavar, Sabina;Jackson, Petra;Wallin, Hakan
通讯作者：
Wallin, Hakan
DOI：
10.1016/0272-0590(90)90171-f
发表时间：
1990-07-01
期刊：
FUNDAMENTAL AND APPLIED TOXICOLOGY
影响因子：
--
作者：
COUTURE, LA;HARRIS, MW;BIRNBAUM, LS
通讯作者：
BIRNBAUM, LS