A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase.
A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase.
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DOI：
10.1021/ja1110514
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发表时间：
2011-06-22
影响因子：
15
通讯作者：
Begley, Tadhg P.
中科院分区：
文献类型：
--
作者：
Hazra, Amrita B.;Han, Ying;Chatterjee, Abhishek;Zhang, Yang;Lai, Rung-Yi;Ealick, Steven E.;Begley, Tadhg P.
在许多细菌中，tenI被发现与硫胺素噻唑生物合成相关的基因成簇。然而，虽然TenI显示出与硫胺素磷酸合酶的高度序列相似性，但纯化的蛋白质没有硫胺素磷酸合酶活性，并且这种酶在硫胺素生物合成中的作用仍然未知。在本文中，我们确定的功能TenI作为噻唑互变异构酶，描述的酶与其反应产物的结构，确定底物磷酸盐和组氨酸122参与催化的酸/碱残基，并提出了一个机制的反应。TenI功能的鉴定完成了主要细菌途径生物合成硫胺素所需的所有酶的鉴定。
In many bacteria tenI is found clustered with genes involved in thiamin thiazole biosynthesis. However, while TenI shows high sequence similarity with thiamin phosphate synthase, the purified protein has no thiamin phosphate synthase activity and the role of this enzyme in thiamin biosynthesis remains unknown. In this paper, we identify the function of TenI as a thiazole tautomerase, describe the structure of the enzyme complexed with its reaction product, identify the substrate phosphate and histidine 122 as the acid/base residues involved in catalysis and propose a mechanism for the reaction. The identification of the function of TenI completes the identification of all of the enzymes needed for thiamin biosynthesis by the major bacterial pathway.
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影响因子：
14.8
作者：
Chatterjee, Abhishek;Li, Yue;Zhang, Yang;Grove, Tyler L.;Lee, Michael;Krebs, Carsten;Booker, Squire J.;Begley, Tadhg P.;Ealick, Steven E.
通讯作者：
Ealick, Steven E.
影响因子：
16.6
作者：
Jurgenson CT;Begley TP;Ealick SE
通讯作者：
Ealick SE
影响因子：
2.9
作者：
Martinez-Gomez, N. Cecilia;Downs, Diana M.
通讯作者：
Downs, Diana M.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
15
作者：
Hazra, Amrita;Chatterjee, Abhishek;Begley, Tadhg P.
通讯作者：
Begley, Tadhg P.