Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.
Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.
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对玉米 (Zea mays) 胚乳中发育的蛋白体进行全面的蛋白质组学分析，为其生物发生提供了新的见解。
DOI：
10.1093/jxb/erw396
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发表时间：
2016-12
影响因子：
6.9
通讯作者：
Song R
中科院分区：
文献类型：
--
作者：
Wang G;Wang G;Wang J;Du Y;Yao D;Shuai B;Han L;Tang Y;Song R
对发育中的玉米蛋白体(PBS)的蛋白质组学分析揭示了PB蛋白出人意料的多样性和复杂性，为玉米醇溶蛋白基因mRNAs的运输和翻译以及PBS的组装提供了路线图。醇溶蛋白是谷物种子的主要储藏蛋白，在内质网(ER)的管腔中被隔离和积累，并直接组装成蛋白体(PBS)。醇溶蛋白的含量和组成是决定籽粒蛋白质品质和质构相关性状的关键因素。同时，PB诱导的融合系统为在植物中生产治疗和工业产品提供了一个有效的靶点。然而，天然PB的蛋白质组及其形成的详细机制仍需确定。我们建立了一种从发育中的玉米胚乳中分离高纯度和完整的PBS的方法，并对玉米醇溶蛋白(Zein)的完整PBS进行了蛋白质组学分析。因此，我们识别了1756种蛋白质，分为五大类：代谢途径、对刺激的反应、运输、发育和生长以及调节。通过比较PBS粗提物和浓缩提取物的蛋白质组，我们发现了以下结论的充分证据：(I)核糖体、ER膜和细胞骨架与形成外围边界的Zein PBS紧密相关；(Ii)Zein RNA可能被运输和定位到PB-ER亚区；以及(Iii)ER伴侣对于Zein折叠、质量控制和组装到PBS是必不可少的。我们进一步证实了OPAQUE1(O1)在酵母中不能直接与FLOURY1(Fl1)相互作用，这表明肌球蛋白XI与含有DUF593的蛋白质之间的相互作用是同种异构体特异性的。这项研究为剖析玉米醇溶蛋白PB的生物发生提供了蛋白质组路线图，并揭示了PBS中蛋白质的意想不到的多样性和复杂性。
Proteomic analysis of developing maize protein bodies (PBs) reveals an unexpected diversity and complexity of PB proteins, and provides a roadmap for the transport and translation of mRNAs of zein genes, and the assembly of PBs. Prolamins, the major cereal seed storage proteins, are sequestered and accumulated in the lumen of the endoplasmic reticulum (ER), and are directly assembled into protein bodies (PBs). The content and composition of prolamins are the key determinants for protein quality and texture-related traits of the grain. Concomitantly, the PB-inducing fusion system provides an efficient target to produce therapeutic and industrial products in plants. However, the proteome of the native PB and the detailed mechanisms underlying its formation still need to be determined. We developed a method to isolate highly purified and intact PBs from developing maize endosperm and conducted proteomic analysis of intact PBs of zein, a class of prolamine protein found in maize. We thus identified 1756 proteins, which fall into five major categories: metabolic pathways, response to stimulus, transport, development, and growth, as well as regulation. By comparing the proteomes of crude and enriched extractions of PBs, we found substantial evidence for the following conclusions: (i) ribosomes, ER membranes, and the cytoskeleton are tightly associated with zein PBs, which form the peripheral border; (ii) zein RNAs are probably transported and localized to the PB–ER subdomain; and (iii) ER chaperones are essential for zein folding, quality control, and assembly into PBs. We futher confirmed that OPAQUE1 (O1) cannot directly interact with FLOURY1 (FL1) in yeast, suggesting that the interaction between myosins XI and DUF593-containing proteins is isoform-specific. This study provides a proteomic roadmap for dissecting zein PB biogenesis and reveals an unexpected diversity and complexity of proteins in PBs.
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影响因子：
5.3
作者：
Joseph M;Ludevid MD;Torrent M;Rofidal V;Tauzin M;Rossignol M;Peltier JB
通讯作者：
Peltier JB
影响因子：
56.9
作者：
LI, XX;WU, YJ;OKITA, TW
通讯作者：
OKITA, TW
影响因子：
11.6
作者：
Gao, Caiji;Yu, Christine K. Y.;Jiang, Liwen
通讯作者：
Jiang, Liwen
影响因子：
6.9
作者：
Ohta M;Wakasa Y;Takahashi H;Hayashi S;Kudo K;Takaiwa F
通讯作者：
Takaiwa F
影响因子：
7.4
作者：
LARKINS, BA;HURKMAN, WJ
通讯作者：
HURKMAN, WJ