UVA irradiation of fatty acids and their oxidized products substantially increases their ability to generate singlet oxygen.
UVA irradiation of fatty acids and their oxidized products substantially increases their ability to generate singlet oxygen.
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脂肪酸及其氧化产物的 UVA 照射大大提高了它们产生单线态氧的能力
DOI：
10.1039/c3cp51399h
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Baeumler W
中科院分区：
文献类型：
--
作者：
Regensburger J;Maisch T;Knak A;Gollmer A;Felgentraeger A;Lehner K;Baeumler W
UVA辐射对人体组织的不良反应起着重要作用。UVA透过皮肤的表皮和真皮被各种生物分子吸收，特别是内源性光敏剂。这可能会产生有害的单线态氧（1 O2），氧化细胞膜、脂蛋白和其他含脂质结构（如表皮屏障）中的脂肪酸。有迹象表明，脂肪酸不仅是1 O2的目标，但也作为潜在的光敏剂在UVA照射下，如果已经氧化。将乙醇溶液中的五种不同脂肪酸（硬脂酸、油酸、亚油酸、亚麻酸和花生四烯酸）暴露于UVA辐射（355 nm，100 mW）30秒。在1270 nm处检测到1 O2发光，并在光谱分辨实验中证实。脂肪酸的双键越多，检测到的1 O2光子越多。此外，脂肪酸连续暴露于宽带UVA长达240分钟。在此期间，UVA吸收和1 O2发光大幅增加，照射时间，达到最大值，并再次下降。HPLC-MS分析表明，过氧化脂肪酸的量和1 O2的产生平行增加和减少。这表明过氧化脂肪酸在UVA照射下产生1 O2的高潜力。总之，脂肪酸沿着过氧化产物是弱内源性光敏剂，但在连续UVA照射下成为强光敏剂。由于脂肪酸及其氧化产物普遍存在于活细胞和皮肤中，而皮肤经常长期暴露于UVA辐射，因此这种光敏效应可能导致组织中有害光氧化过程的启动。
UVA radiation plays an important role for adverse reactions in human tissue. UVA penetrates epidermis and dermis of skin being absorbed by various biomolecules, especially endogenous photosensitizers. This may generate deleterious singlet oxygen (1O2) that oxidizes fatty acids in cell membranes, lipoproteins, and other lipid-containing structures such as the epidermal barrier. Indications exist that fatty acids are not only the target of 1O2 but also act as potential photosensitizers under UVA irradiation, if already oxidized. Five different fatty acids in ethanol solution (stearic, oleic, linoleic, linolenic and arachidonic acid) were exposed to UVA radiation (355 nm, 100 mW) for 30 seconds. 1O2 luminescence was detected time-resolved at 1270 nm and confirmed in spectrally-resolved experiments. The more double bonds fatty acids have the more 1O2 photons were detected. In addition, fatty acids were continuously exposed to broadband UVA for up to 240 min. During that time span, UVA absorption and 1O2 luminescence substantially increased with irradiation time, reached a maximum and decreased again. HPLC-MS analysis showed that the amount of peroxidized fatty acids and the 1O2 generation increased and decreased in parallel. This indicates the high potential of peroxidized fatty acids to produce 1O2 under UVA irradiation. In conclusion, fatty acids along with peroxidized products are weak endogenous photosensitizers but become strong photosensitizers under continuous UVA irradiation. Since fatty acids and their oxidized products are ubiquitous in living cells and in skin, which is frequently and long-lasting exposed to UVA radiation, this photosensitizing effect may contribute to initiation of deleterious photooxidative processes in tissue.
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影响因子：
7.4
作者：
Bando, N;Hayashi, H;Terao, J
通讯作者：
Terao, J
紫外线影响细胞膜和细胞质目标。
DOI：
--
发表时间：
1998
期刊：
Journal of Photochemistry and Photobiology. B: Biology
影响因子：
--
作者：
Thomas Schwarz
通讯作者：
Thomas Schwarz
9-乙酰氧基-2,7,12,17-四-(β-甲氧基乙基)-卟啉 (ATMPn) 在溶液中产生单线态氧
DOI：
10.1016/s0301-0104(02)00806-6
发表时间：
2002
期刊：
Journal of photochemistry and photobiology. B, Biology
影响因子：
--
作者：
D. Baumer;M. Maier;R. Engl;R. Szeimies;W. Bäumler
通讯作者：
W. Bäumler
影响因子：
3.5
作者：
Baier, Juergen;Maisch, Tim;Baeumler, Wolfgang
通讯作者：
Baeumler, Wolfgang
影响因子：
6.5
作者：
Lampe,MA;Williams,ML;Elias,PM
通讯作者：
Elias,PM