Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6.
Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6.
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新的人类乳腺癌细胞系KPL-4的隔离和表征，表达ERB B家族受体和白介素6。
DOI：
10.1038/sj.bjc.6690114
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发表时间：
1999-02
影响因子：
8.8
通讯作者：
Sonoo, H
中科院分区：
文献类型：
--
作者：
Kurebayashi, J;Otsuki, T;Tang, CK;Kurosumi, M;Yamamoto, S;Tanaka, K;Mochizuki, M;Nakamura, H;Sonoo, H
一种新的人乳腺癌细胞系，KPL-4，最近从患有炎性皮肤转移的乳腺癌患者的恶性胸腔积液中分离出来。该细胞系可在无血清条件下培养，在雌性无胸腺裸鼠中具有致瘤性。流式细胞仪分析显示ErB B-1、-2和-3的表达。斑点杂交显示erbB-2扩增了15倍。逆转录-聚合酶链反应分析显示所有ErB B家族受体的mRNA表达的可检测水平。此外，所有的受体在血清补充条件下自磷酸化。出乎意料的是，移植的KPL-4肿瘤诱导了受体小鼠的恶病质。在培养液和小鼠血清中均检测到高浓度的白细胞介素-6（IL-6）。肿瘤重量与血清IL-6水平显著相关。研究了人源化抗ErB B-2单克隆抗体rhuMAbHER 2的抗增殖作用。该抗体在体外显著抑制KPL-4细胞的生长，但在体内适度抑制。rhuMAbHER 2可部分逆转小鼠体重减轻。这些发现表明，KPL-4细胞可能是有用的，在开发新的策略，对乳腺癌过表达的ErB B家族受体和对IL-6诱导的恶病质。© 1999癌症研究运动
A new human breast cancer cell line, KPL-4, was recently isolated from the malignant pleural effusion of a breast cancer patient with an inflammatory skin metastasis. This cell line can be cultured under serum-free conditions and is tumorigenic in female athymic nude mice. Flow cytometric analysis revealed the expression of Erb B-1, -2 and -3. Dot blot hybridization showed a 15-fold amplification of the erbB-2. Reverse transcription-polymerase chain reaction analysis showed a detectable level of mRNA expression of all the Erb B family receptors. In addition, all the receptors were autophosphorylated under a serum-supplemented condition. Unexpectedly, transplanted KPL-4 tumours induced cachexia of recipient mice. A high concentration of interleukin-6 (IL-6) was detected in both the culture medium and the serum of mice. The weight of tumours significantly correlated with the serum IL-6 level. The antiproliferative effect of a humanized anti-Erb B-2 monoclonal antibody, rhuMAbHER2, was investigated. This antibody significantly inhibited the growth of KPL-4 cells in vitro but modestly in vivo. Loss of mouse body weight was partly reversed by rhuMAbHER2. These findings suggest that KPL-4 cells may be useful in the development of new strategies against breast cancer overexpressing the Erb B family receptors and against IL-6-induced cachexia. © 1999 Cancer Research Campaign
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一种新的人类乳腺癌细胞系 KPL-3C 分泌甲状旁腺激素相关蛋白，并在裸鼠体内产生与微钙化相关的肿瘤。
影响因子：
8.8
作者：
Kurebayashi, J;Kurosumi, M;Sonoo, H
通讯作者：
Sonoo, H
影响因子：
11.4
作者：
GrausPorta, D;Beerli, RR;Hynes, NE
通讯作者：
Hynes, NE
影响因子：
11.4
作者：
Alimandi, M;Wang, LM;Pierce, JH
通讯作者：
Pierce, JH
DOI：
10.1073/pnas.90.5.1746
发表时间：
1993-03-01
影响因子：
11.1
作者：
PLOWMAN, GD;CULOUSCOU, JM;SHOYAB, M
通讯作者：
SHOYAB, M
DOI：
10.1073/pnas.89.10.4285
发表时间：
1992-05-15
影响因子：
11.1
作者：
CARTER, P;PRESTA, L;SHEPARD, HM
通讯作者：
SHEPARD, HM