A new human breast cancer cell line, KPL-3C, secretes parathyroid hormone-related protein and produces tumours associated with microcalcifications in nude mice.
A new human breast cancer cell line, KPL-3C, secretes parathyroid hormone-related protein and produces tumours associated with microcalcifications in nude mice.
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一种新的人类乳腺癌细胞系 KPL-3C 分泌甲状旁腺激素相关蛋白，并在裸鼠体内产生与微钙化相关的肿瘤。
DOI：
10.1038/bjc.1996.338
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发表时间：
1996-07
影响因子：
8.8
通讯作者：
Sonoo, H
中科院分区：
文献类型：
--
作者：
Kurebayashi, J;Kurosumi, M;Sonoo, H
甲状旁腺素相关蛋白（PTHrP）是恶性肿瘤体液性高钙血症（HHM）的主要原因。我们最近建立了一个新的人乳腺癌细胞系，命名为KPL-3C，从乳腺癌患者的恶性积液HHM。形态学、细胞遗传学和免疫组织化学分析表明，该细胞系来源于人乳腺癌。KPL-3C细胞稳定分泌免疫反应性PTHrP测量的两个网站的免疫放射分析，具有雌激素和孕激素受体，并在雌性裸鼠的致瘤性。向培养基中添加佛波醇-12-肉豆蔻酸酯-13-乙酸酯显著增加了细胞的PTHrP分泌。与此相反，氢化可的松，醋酸甲羟孕酮和22-oxacalcitriol减少PTHrP分泌的剂量依赖性方式。出乎意料的是，在移植的肿瘤中观察到许多微钙化。放射学检查表明，肿瘤中的微钙化与人类乳腺癌中常见的微钙化非常相似。这些发现表明，这种KPL-3C细胞系可能是有用的研究PTHrP分泌的调节机制和机制，导致微钙化的沉积在乳腺癌。
Parathyroid hormone-related protein (PTHrP) is the main cause of humoral hypercalcaemia of malignancy (HHM). We recently established a new human breast cancer cell line, designated KPL-3C, from the malignant effusion of a breast cancer patient with HHM. Morphological, cytogenetic and immunohistochemical analyses indicated that the cell line is derived from human breast cancer. The KPL-3C cells stably secrete immunoreactive PTHrP measured by a two-site immunoradiometric assay, possess both oestrogen and progesterone receptors and are tumorigenic in female nude mice. The addition of phorbol-12-myristate-13-acetate to the medium significantly increased PTHrP secretion from the cells. In contrast, hydrocortisone, medroxyprogesterone acetate and 22-oxacalcitriol decreased PTHrP secretion in a dose-dependent manner. Unexpectedly, a number of microcalcifications were observed in the transplanted tumours. Radiographical examination indicated that the microcalcifications in the tumours are very similar to those commonly observed in human breast cancer. These findings suggest that this KPL-3C cell line may be useful for studying the regulatory mechanisms of PTHrP secretion and the mechanisms that lead to the deposition of microcalcifications in breast cancer.
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影响因子：
8.8
作者：
Birch MA;Carron JA;Scott M;Fraser WD;Gallagher JA
通讯作者：
Gallagher JA
影响因子：
5.8
作者：
IKEDA, K;OHNO, H;MATSUMOTO, T
通讯作者：
MATSUMOTO, T
DOI：
10.1007/bf02032890
发表时间：
1994-01-01
期刊：
SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY
影响因子：
--
作者：
KOHNO, N;KITAZAWA, S;SAITOH, Y
通讯作者：
SAITOH, Y
影响因子：
4.8
作者：
MERRYMAN, JI;ROSOL, TJ;CAPEN, CC
通讯作者：
CAPEN, CC
影响因子：
19.7
作者：
DELAFONTAN, B;DAURES, JP;PUJOL, H
通讯作者：
PUJOL, H