Patterns of mRNA and protein expression for 12 GABAA receptor subunits in the mouse brain.
Patterns of mRNA and protein expression for 12 GABAA receptor subunits in the mouse brain.
复制标题
DOI：
10.1016/j.neuroscience.2013.01.008
复制
发表时间：
2013-04-16
期刊：
影响因子：
3.3
通讯作者：
Sperk G
中科院分区：
文献类型：
--
作者：
Hörtnagl H;Tasan RO;Wieselthaler A;Kirchmair E;Sieghart W;Sperk G
GABAA受体亚单位的分布是高度异质性的。mRNA的分布与蛋白质的分布相对应。小鼠中的分布与大鼠中的分布基本相关，但存在明显差异。GABAA受体是脑中的主要抑制性受体，其亚基来源于不同的基因或基因家族（α1-α6、β1-β3、γ1-γ3、δ、ε、θ、π或ρ1-3）。在小鼠脑中，通过原位杂交（ISH）评估，到目前为止研究的GABAA受体亚基mRNA的解剖学分布仅限于前脑和中脑中形成苯二氮卓类敏感受体复合物（α1-α3、α5、β2、β3和γ2）的亚基。本研究应用原位杂交和免疫组织化学方法分析了GABAA受体亚单位α1-α6、β1-β3、γ1-γ2和δ在小鼠脑（不包括脑干）中的解剖分布。在几个脑区，如海马，小脑，嗅球和缰，我们观察到mRNA水平并不反映蛋白质水平，表明蛋白质位于远离细胞体。我们还比较了这12个亚基mRNA和蛋白质的分布与大鼠脑中的报告。虽然一般来说，小鼠和大鼠大脑之间的分布有相当大的对应关系，但观察到几个种属特异性差异。
► The distribution of GABAA receptor subunits is highly heterogeneous. ► The distribution of mRNAs corresponds to that of proteins. ► The distribution in the mouse correlates largely to that in rats although there are distinct differences. The GABAA receptor is the main inhibitory receptor in the brain and its subunits originate from different genes or gene families (α1–α6, β1–β3, γ1–γ3, δ, ε, θ, π, or ρ1–3). In the mouse brain the anatomical distribution of GABAA receptor subunit mRNAs so far investigated is restricted to subunits forming benzodiazepine-sensitive receptor complexes (α1–α3, α5, β2, β3 and γ2) in the forebrain and midbrain as assessed by in situ hybridization (ISH). In the present study the anatomical distribution of the GABAA receptor subunits α1–α6, β1–β3, γ1–γ2 and δ was analyzed in the mouse brain (excluding brain stem) by ISH and immunohistochemistry (IHC). In several brain areas such as hippocampus, cerebellum, bulbus olfactorius and habenula we observed that mRNA levels did not reflect protein levels, indicating that the protein is located far distantly from the cell body. We also compared the distribution of these 12 subunit mRNAs and proteins with that reported in the rat brain. Although in general there is a considerable correspondence in the distribution between mouse and rat brains, several species-specific differences were observed.
登录
查看更多内容
影响因子：
3.3
作者：
Heldt, S. A.;Ressler, K. J.
通讯作者：
Ressler, K. J.
DOI：
10.1073/pnas.96.22.12905
发表时间：
1999-10-26
影响因子：
11.1
作者：
Mihalek, RM;Banerjee, PK;Homanics, GE
通讯作者：
Homanics, GE
影响因子：
2.5
作者：
Peng, Z;Hauer, B;Houser, CR
通讯作者：
Houser, CR
影响因子：
5.3
作者：
Feng, HJ;Kang, JQ;Macdonald, RL
通讯作者：
Macdonald, RL
影响因子：
1.9
作者：
Lu, Yang;Wang, Xuefeng
通讯作者：
Wang, Xuefeng