Downregulation of miRNA miR-1305 and upregulation of miRNA miR-6785-5p may be associated with psoriasis.
Downregulation of miRNA miR-1305 and upregulation of miRNA miR-6785-5p may be associated with psoriasis.
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DOI：
10.3389/fgene.2022.891465
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发表时间：
2022
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
背景：血清细胞外小泡(EVS)在银屑病中的作用鲜为人知。目的：探讨银屑病患者血清血管内皮生长因子(EVS)的转录谱及其潜在的生物标志物。方法：采用差速超速离心法分离EVS，并用透射电子显微镜进行鉴定。用纳米粒子跟踪分析检测电动汽车的直径。共聚焦荧光显微镜观察血清EVS与角质形成细胞的相互作用。分别对4例血清EVS和3例皮损组织进行miRNA芯片和mRNA芯片检测。采用定量逆转录聚合酶链式反应(qRT-PCR)和荧光原位杂交技术检测15例患者血清EVS和皮损组织中miRNAs的表达。通过生物信息学分析预测miR-1305和miR-6785-5p的潜在靶基因和功能。用Western印迹、CCK-8和酶联免疫吸附试验(ELISA)检测EV的生物标志物、角质形成细胞增殖和细胞因子的分泌。结果：共有16个miRNAs和1,725个mRNAs在血清EVS和皮损中显著异常。在血清EVS和皮损中，miR-1305下调，miR-6785-5p上调。血清EVS可被角质形成细胞摄取。与对照组比较，银屑病血清EVS作用后角质形成细胞MIR-1305表达下调，miR-6785-5p表达上调。银屑病患者血清EVS促进角质形成细胞增殖，促进CCL20和IL-8的分泌。血清EVS、miR-1305和miR-6785-5p与疾病严重程度相关。结论：银屑病患者血清EVS可能通过负载miRNAs参与角质形成细胞的活化。血清EVS、miR-1305和miR-6785-5p可能与银屑病有关。
Background: The role of serum extracellular vesicles (EVs) is less known in psoriasis. Objectives: To explore the transcriptomic profile of serum EVs and the potential biomarkers in psoriasis. Methods: EVs were isolated by differential ultracentrifugation and identified by transmission electron microscope. The diameters of EVs were detected using nanoparticle tracking analysis. Serum EVs-keratinocyte interaction was observed through confocal fluorescence microscopy. miRNA microarray and mRNA microarray were performed in serum EVs (n = 4) and skin lesions (n = 3), respectively. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and fluorescence in situ hybridization were used to detect the expression of miRNAs in serum EVs and skin lesions (n = 15). Bioinformatics analysis was performed to predict the potential target genes and functions of miR-1305 and miR-6785-5p. Western blot, CCK-8 and enzyme-linked immunosorbent assay (ELISA) were used to detect the EVs’ biomarkers, keratinocytes proliferation and cytokines secretion. Results: A total of 16 miRNAs and 1,725 mRNAs were significantly dysregulated in serum EVs and skin lesions, respectively. miR-1305 was down-regulated and miR-6785-5p was upregulated in both serum EVs and skin lesions. Serum EVs could be taken up by keratinocytes. miR-1305 was downregulated and miR-6785-5p were upregulated in keratinocytes after co-cultured with psoriasis serum EVs compared with controls. Psoriasis serum EVs promoted keratinocyte proliferation and the secretion of CCL20 and IL-8. Serum EVs miR-1305 and miR-6785-5p were associated with disease severity. Conclusion: Serum EVs might be involved in the activation of keratinocytes through loaded miRNAs in psoriasis. Serum EVs miR-1305 and miR-6785-5p may be associated with psoriasis.
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DOI：
10.1002/wrna.1413
发表时间：
2017-07
期刊：
Wiley interdisciplinary reviews. RNA
影响因子：
--
作者：
Kim KM;Abdelmohsen K;Mustapic M;Kapogiannis D;Gorospe M
通讯作者：
Gorospe M
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影响因子：
3.6
作者：
Jiang, Meng;Ma, Weiyuan;Sun, Qing
通讯作者：
Sun, Qing
影响因子：
17.1
作者：
Guinea-Viniegra, Juan;Jimenez, Maria;Wagner, Erwin F.
通讯作者：
Wagner, Erwin F.
DOI：
10.1111/jdv.16203
发表时间：
2020-02-27
影响因子：
9.2
作者：
Pasquali, L.;Svedbom, A.;Sonkoly, E.
通讯作者：
Sonkoly, E.
DOI：
10.1084/jem.20160258
发表时间：
2016-10-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Cheung KL;Jarrett R;Subramaniam S;Salimi M;Gutowska-Owsiak D;Chen YL;Hardman C;Xue L;Cerundolo V;Ogg G
通讯作者：
Ogg G