Upconversion optogenetic micro-nanosystem optically controls the secretion of light-responsive bacteria for systemic immunity regulation.
Upconversion optogenetic micro-nanosystem optically controls the secretion of light-responsive bacteria for systemic immunity regulation.
复制标题
上转换光遗传学微纳米系统光学控制光响应细菌的分泌以实现全身免疫调节
DOI：
10.1038/s42003-020-01287-4
复制
发表时间：
2020-10-09
影响因子：
5.9
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Yang C;Cui M;Zhang Y;Pan H;Liu J;Wang S;Ma N;Chang J;Sun T;Wang H
肠道微生物群特异性分泌到血液和目标组织中的化学分子可以有效地影响肠道的相关功能，特别是免疫功能，代表了免疫相关疾病的新策略。然而，调节特定菌株分泌代谢的合适方法仍有待建立。本文构建了一个上转换光遗传微纳系统，以有效调节工程细菌的特异性分泌。该系统包括两个主要模块:(i)修饰分泌性光反应工程细菌。（ii）上转换光遗传微纳系统介导的光传感。该系统可以通过光学操作调节工程细菌免疫因子的高效分泌。选择炎症性肠病和皮下移植肿瘤来验证该系统的有效性。我们的研究结果表明，内源性因子TGF-β1可以可控地分泌，抑制肠道炎症反应。此外，促进IFN-γ的调节性分泌以减缓B16F10肿瘤的进展。
Chemical molecules specifically secreted into the blood and targeted tissues by intestinal microbiota can effectively affect the associated functions of the intestine especially immunity, representing a new strategy for immune-related diseases. However, proper ways of regulating the secretion metabolism of specific strains still remain to be established. In this article, an upconversion optogenetic micro-nanosystem was constructed to effectively regulate the specific secretion of engineered bacteria. The system included two major modules: (i) Modification of secretory light-responsive engineered bacteria. (ii) Optical sensing mediated by upconversion optogenetic micro-nanosystem. This system could regulate the efficient secretion of immune factors by engineered bacteria through optical manipulation. Inflammatory bowel disease and subcutaneously transplanted tumors were selected to verify the effectiveness of the system. Our results showed that the endogenous factor TGF-β1 could be controllably secreted to suppress the intestinal inflammatory response. Additionally, regulatory secretion of IFN-γ was promoted to slow the progression of B16F10 tumor.
登录
查看更多内容
影响因子：
24.8
作者：
Knoop KA;Gustafsson JK;McDonald KG;Kulkarni DH;Coughlin PE;McCrate S;Kim D;Hsieh CS;Hogan SP;Elson CO;Tarr PI;Newberry RD
通讯作者：
Newberry RD
影响因子：
48
作者：
Berlin S;Carroll EC;Newman ZL;Okada HO;Quinn CM;Kallman B;Rockwell NC;Martin SS;Lagarias JC;Isacoff EY
通讯作者：
Isacoff EY
影响因子：
7
作者：
Cook, Michael T.;Tzortzis, George;Charalampopoulos, Dimitris
通讯作者：
Charalampopoulos, Dimitris
影响因子：
64.8
作者：
Mao, Kairui;Baptista, Antonio P.;Germain, Ronald N.
通讯作者：
Germain, Ronald N.
肠道粘蛋白型 O-糖基化缺陷导致小鼠自发性结肠炎相关癌症。
影响因子：
29.4
作者：
Bergstrom K;Liu X;Zhao Y;Gao N;Wu Q;Song K;Cui Y;Li Y;McDaniel JM;McGee S;Chen W;Huycke MM;Houchen CW;Zenewicz LA;West CM;Chen H;Braun J;Fu J;Xia L
通讯作者：
Xia L