Genetic and cellular characterization of MscS-like putative channels in the filamentous fungus Aspergillus nidulans.
Genetic and cellular characterization of MscS-like putative channels in the filamentous fungus Aspergillus nidulans.
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DOI：
10.1080/19336950.2022.2098661
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发表时间：
2022-12
期刊：
影响因子：
3.3
通讯作者：
Diallinas, George
中科院分区：
文献类型：
--
作者：
Dionysopoulou, Mariangela;Yan, Nana;Wang, Bolin;Pliotas, Christos;Diallinas, George
机械敏感性离子通道是普遍存在于细菌、古细菌和真核生物中的膜蛋白。它们充当机械应力的分子传感器，以提供诸如触觉、听觉、渗透压、本体感受和平衡等重要功能，而它们的功能障碍通常与病理学有关。其中，来自细菌的结构上不同的MscL和MscS通道是研究最广泛的。在植物和粟酒裂殖酵母中发现了MscS样通道，在低渗条件下它们调节细胞内Ca 2+和细胞体积。在这里，我们描述了两个MSCS样推定的通道，命名为MSCA和MSCB，从模型丝状真菌构巢曲霉。MscA和MscB的直系同源物存在于大多数真菌中，包括相关的植物和动物病原体。MscA/MscB和其他真菌MscS样蛋白共享三个跨膜螺旋和延伸的C-末端胞质结构域，其形成MscS样通道的结构指纹，与大肠杆菌MscS相比具有至少三个额外的跨膜区段。我们发现，MscA和MscB定位在内质网和质膜，分别，而它们的过度表达导致氯化钙的毒性增加或/和减少无性孢子的形成。我们的研究结果有助于了解MscS-like通道在丝状真菌和相关病原体中的作用。
Mechanosensitive ion channels are integral membrane proteins ubiquitously present in bacteria, archaea, and eukarya. They act as molecular sensors of mechanical stress to serve vital functions such as touch, hearing, osmotic pressure, proprioception and balance, while their malfunction is often associated with pathologies. Amongst them, the structurally distinct MscL and MscS channels from bacteria are the most extensively studied. MscS-like channels have been found in plants and Schizosaccharomyces pombe, where they regulate intracellular Ca2+ and cell volume under hypo-osmotic conditions. Here we characterize two MscS-like putative channels, named MscA and MscB, from the model filamentous fungus Aspergillus nidulans. Orthologues of MscA and MscB are present in most fungi, including relative plant and animal pathogens. MscA/MscB and other fungal MscS-like proteins share the three transmembrane helices and the extended C-terminal cytosolic domain that form the structural fingerprint of MscS-like channels with at least three additional transmembrane segments than Escherichia coli MscS. We show that MscA and MscB localize in Endoplasmic Reticulum and the Plasma Membrane, respectively, whereas their overexpression leads to increased CaCl2 toxicity or/and reduction of asexual spore formation. Our findings contribute to understanding the role of MscS-like channels in filamentous fungi and relative pathogens.
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影响因子：
2.9
作者：
Peyronnet, Remi;Haswell, Elizabeth S.;Frachisse, Jean-Marie
通讯作者：
Frachisse, Jean-Marie
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
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影响因子：
16.6
作者：
Deng, Zengqin;Maksaev, Grigory;Yuan, Peng
通讯作者：
Yuan, Peng
DOI：
10.4161/chan.20998
发表时间：
2012-07
期刊：
Channels (Austin, Tex.)
影响因子：
--
作者：
Edwards MD;Black S;Rasmussen T;Rasmussen A;Stokes NR;Stephen TL;Miller S;Booth IR
通讯作者：
Booth IR
影响因子：
11.4
作者：
Li, YZ;Moe, PC;Blount, P
通讯作者：
Blount, P