Inference of gene regulation via miRNAs during ES cell differentiation using MiRaGE method.

Inference of gene regulation via miRNAs during ES cell differentiation using MiRaGE method.
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DOI:
10.3390/ijms12129265
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发表时间:
2011
影响因子:
5.6
通讯作者:
Yasuda J
Yasuda J
中科院分区:
生物学2区
文献类型:
--
作者:
Yoshizawa M;Taguchi YH;Yasuda J

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MicroRNA (miRNA)是细胞生长、分化和发育的关键调控因子。为了识别生物过程中重要的mirna,已经开发了许多生物信息学工具。我们开发了MiRaGE (MiRNA Ranking by Gene Expression)方法,从基因表达谱的变化推断MiRNA对基因表达的调控。该方法不需要先前的数组规范化。我们应用该方法来阐明胚胎干细胞向神经细胞分化过程中可能重要的mirna,我们推断某些mirna,包括miR-200家族、miR-429、miR-302家族和miR-17-92集群成员可能对维持胚胎干细胞的未分化状态很重要。
MicroRNA (miRNA) is a critical regulator of cell growth, differentiation, and development. To identify important miRNAs in a biological process, many bioinformatical tools have been developed. We have developed MiRaGE (MiRNA Ranking by Gene Expression) method to infer the regulation of gene expression by miRNAs from changes of gene expression profiles. The method does not require precedent array normalization. We applied the method to elucidate possibly important miRNAs during embryonic stem (ES) cell differentiation to neuronal cells and we infer that certain miRNAs, including miR-200 family, miR-429, miR-302 family, and miR-17-92 cluster members may be important to the maintenance of undifferentiated status in ES cells.
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