Cytokine-Induced Killer Cells Modulates Resistance to Cisplatin in the A549/DDP Cell Line.
Cytokine-Induced Killer Cells Modulates Resistance to Cisplatin in the A549/DDP Cell Line.
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细胞因子诱导的杀伤细胞调节 A549/DDP 细胞系对顺铂的耐药性
DOI：
10.7150/jca.19426
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发表时间：
2017
影响因子：
3.9
通讯作者：
Ren X
中科院分区：
文献类型：
--
作者：
Yang L;Du C;Wu L;Yu J;An X;Yu W;Cao S;Li H;Ren X
研究背景细胞因子诱导的杀伤细胞（CIK）能增强化疗的肿瘤杀伤活性。目的探讨CIK细胞对人肺腺癌细胞系A549/DDP顺铂耐药的影响。方法体外检测A549/DDP与CIK细胞共培养后的耐药指数、耐药相关基因及细胞因子分泌情况。结果A549/DDP与CIK细胞共培养后，A549/DDP对DDP的耐药性明显降低，并呈时间依赖性。CIK细胞与A549/DDP共培养20 h后，A549/DDP细胞对DDP的耐药性较单独培养时下降4.93倍（P<0.05）。与CIK细胞共培养后，A549/DDP细胞GST-π基因的mRNA和蛋白表达水平均显著降低（P<0.05）。随着A549/DDP与CIK细胞共培养时间的延长，IFN- γ的分泌量沿着增加。加入中和性IFN-γ后，GST-π的表达恢复。结论CIK细胞可通过降低GST-π的表达，增加DDP的蓄积，逆转A549/DDP的耐药性，并呈时间依赖性。CIK细胞对肺癌细胞化疗药物的增敏作用部分依赖于IFN-γ的分泌。
Background Cytokine-induced killer (CIK) cells can potentially enhance the tumor-killing activity of chemotherapy. Objective This study aimed to evaluate the effects of CIK cells on cisplatin (DDP) resistance in the human lung adenocarcinoma cell line A549/DDP. Methods The detect resistance index, drug resistance related-genes and cytokine secretion of A549/DDP co-cultured with CIK cells were assayed in vitro. ResultsAfter A549/DDP co-culture with CIK cells, the DDP resistance of A549/DDP significantly decreased in a time-dependent manner. The DDP resistance of A549/DDP co-cultured with CIK cells for 20 h decreased 4.93-fold compared with that of A549/DDP cells cultured alone (P<0.05). The mRNA and protein expression levels of the glutathione-S-transferase (GST) -π gene in A549/DDP significantly decreased after co-culture with CIK cells (P<0.05). The secretion of interferon (IFN)- γ significantly increased along with the co-culture time of A549/DDP with CIK cells. The expression of GST-π was restored by adding the neutralizing IFN-γ. ConclusionCIK cells can reverse the drug resistance of A549/DDP in a time-dependent manner by reducing GST-π expression to increase the accumulation of DDP. The effect of CIK cells on re-sensitizing lung cancer cells to the chemotherapy drug was partially dependent on the secretion of IFN-γ.
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影响因子：
2.6
作者：
Geng M;Wang L;Chen X;Cao R;Li P
通讯作者：
Li P
影响因子：
11.2
作者：
Federici, Luca;Lo Sterzo, Carlo;Caccuri, Anna Maria
通讯作者：
Caccuri, Anna Maria
影响因子：
3.4
作者：
Liu, Pengying;Chen, Longbang;Huang, Xiang
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影响因子：
3.4
作者：
Ren, XB;Yu, JP;Hao, XS
通讯作者：
Hao, XS
影响因子：
11.5
作者：
Lagas, Jurjen S.;van Waterschoot, Robert A. B.;Schinkel, Alfred H.
通讯作者：
Schinkel, Alfred H.