The association between chemosensitivity and Pgp, GST-π and Topo II expression in gastric cancer.
The association between chemosensitivity and Pgp, GST-π and Topo II expression in gastric cancer.
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DOI：
10.1186/1746-1596-8-198
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发表时间：
2013-12-10
影响因子：
2.6
通讯作者：
Li P
中科院分区：
文献类型：
--
作者：
Geng M;Wang L;Chen X;Cao R;Li P
探讨P-糖蛋白（Pgp）、谷胱甘肽S-转移酶π（GST-π）和拓扑异构酶II（Topo II）表达与人胃癌体外化疗耐药的关系。原代单细胞悬浮液由原发性胃癌的新鲜标本制备，并暴露于羟基喜树碱 (HCPT)、顺铂 (CDDP)、5-氟尿嘧啶 (5-FU)、阿霉素 (ADM) 和丝裂霉素 (MMC) 48 小时。使用四唑（MTT）测定评估细胞代谢活性和抑制率。使用免疫组织化学测定胃癌组织样本中的 Pgp、GST-π 和 Topo II 表达。胃癌细胞的化疗敏感性各不相同； HCPT、CDDP和5-FU暴露的细胞抑制率显着高于ADM和MMC暴露的细胞(p<0.05)。表达 Pgp 的胃癌细胞对 ADM 和 HCPT 具有耐药性（分别为 p = 0.008 和 p = 0.011）。对 5-FU、CDDP 和 MMC 耐药的细胞具有显着较高的 GST-π 表达 (p < 0.05)。在对 HCPT、ADM 和 MMC 耐药的细胞中，Topo II 表达显着降低 (p<<0.05)。 Pgp和GST-π表达可能有助于胃癌细胞对某些化疗药物的原发性耐药，而Topo II表达可能表明HCPT、ADM和MMC敏感性。 Pgp、GST-π 和 Topo II 检测以及 MTT 测定可用作化疗药物给药和胃癌耐药性识别的预测因子。本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/3448329111142964。
To investigate the relationship between P-glycoprotein (Pgp), glutathione S-transferase π (GST-π) and topoisomerase II (Topo II) expression and human gastric cancer chemoresistance in vitro. Primary single-cell suspensions were prepared from fresh specimens of primary gastric cancer and exposed to hydroxycamptothecin (HCPT), cisplatin (CDDP), 5-fluorouracil (5-FU), adriamycin (ADM) and mitomycin (MMC) for 48 h. Cell metabolic activity and rate of inhibition were evaluated using tetrazolium (MTT) assay. Pgp, GST-π and Topo II expression was determined in gastric carcinoma tissue samples using immunohistochemistry. Chemosensitivity of the gastric cancer cells varied; the rates of inhibition of cells exposed to HCPT, CDDP and 5-FU were significantly higher than that of cells exposed to ADM and MMC (p < 0.05). Gastric cancer cells with Pgp expression were resistant to ADM and HCPT (p = 0.008 and p = 0.011, respectively). Cells resistant to 5-FU, CDDP and MMC had significantly higher GST-π expression (p < 0.05). Topo II expression was significantly lower in cells resistant to HCPT, ADM and MMC (p < 0.05). Pgp and GST-π expression may contribute to primary resistance of gastric cancer cells to some chemotherapeutic drugs, while Topo II expression may indicate HCPT, ADM and MMC sensitivity. Pgp, GST-π and Topo II detection and the MTT assay could be used as predictors in chemotherapeutic drug administration and for identifying drug resistance in gastric carcinoma. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3448329111142964.
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