Dissecting the M phase-specific phosphorylation of serine-proline or threonine-proline motifs.
Dissecting the M phase-specific phosphorylation of serine-proline or threonine-proline motifs.
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DOI：
10.1091/mbc.e09-06-0486
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发表时间：
2010-05-01
影响因子：
3.3
通讯作者：
Kuang J
中科院分区：
文献类型：
--
作者：
Wu CF;Wang R;Liang Q;Liang J;Li W;Jung SY;Qin J;Lin SH;Kuang J
Mitotic induction tightly associates with increased reactivity to mitotic phosphoprotein antibody MPM-2. Here we report that phosphorylation of TP motifs surrounded by hydrophobic residues at the −1 and +1 positions plays a dominant role in M phase–associated MPM-2 reactivity. The majority of these motifs are not phosphorylated by mitotic Cdk or MAPK. M phase induction in eukaryotic cell cycles is associated with a burst of protein phosphorylation, primarily at serine or threonine followed by proline (S/TP motif). The mitotic phosphoprotein antibody MPM-2 recognizes a significant subset of mitotically phosphorylated S/TP motifs; however, the required surrounding sequences of and the key kinases that phosphorylate these S/TP motifs remain to be determined. By mapping the mitotic MPM-2 epitopes in Xenopus Cdc25C and characterizing the mitotic MPM-2 epitope kinases in Xenopus oocytes and egg extracts, we have determined that phosphorylation of TP motifs that are surrounded by hydrophobic residues at both −1 and +1 positions plays a dominant role in M phase–associated burst of MPM-2 reactivity. Although mitotic Cdk and MAPK may phosphorylate subsets of these motifs that have a basic residue at the +2 position and a proline residue at the −2 position, respectively, the majority of these motifs that are preferentially phosphorylated in mitosis do not have these features. The M phase–associated burst of MPM-2 reactivity can be induced in Xenopus oocytes and egg extracts in the absence of MAPK or Cdc2 activity. These findings indicate that the M phase–associated burst of MPM-2 reactivity represents a novel type of protein phosphorylation in mitotic regulation.
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影响因子：
7.5
作者：
Kelly AE;Funabiki H
通讯作者：
Funabiki H
影响因子：
64.5
作者：
KING, RW;PETERS, JM;KIRSCHNER, MW
通讯作者：
KIRSCHNER, MW
影响因子：
4.8
作者：
Himpel, S;Tegge, W;Becker, W
通讯作者：
Becker, W
影响因子：
3.3
作者：
IZUMI, T;MALLER, JL
通讯作者：
MALLER, JL
DOI：
10.1073/pnas.80.10.2926
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DAVIS, FM;TSAO, TY;RAO, PN
通讯作者：
RAO, PN