Effects of varied fatigue protocols on sarcoplasmic reticulum calcium uptake and release rates.

Effects of varied fatigue protocols on sarcoplasmic reticulum calcium uptake and release rates.
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不同疲劳方案对肌浆网钙摄取和释放率的影响。

DOI:
10.1152/ajpregu.1998.275.1.r99
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发表时间:
1998
期刊:
The American journal of physiology
影响因子:
--
通讯作者:
Williams,JH
Williams,JH
中科院分区:
--
文献类型:
--
作者:
Ward,CW;Spangenburg,EE;Diss,LM;Williams,JH

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The purpose of this investigation was to examine changes in sarcoplasmic reticulum (SR) function in muscles subjected to different patterns of muscle activity. Frog sartorius muscles were stimulated with tetanic trains (100 ms, 100 Hz) delivered at rates of 2.0, 0.5, and 0.2 trains/s. In one set of experiments, stimulation was continued until force had declined to ∼17% of initial (constant fatigue), whereas in the other set, stimulation was continued for 1 min (constant duration). In the constant-fatigue experiments, Ca2+uptake (1 mM MgATP) and release rates (25 μM AgNO3, 5 mM 4-chloro-m-cresol) were depressed by similar extents following each protocol. This occurred despite 1, 4, and 17 min of stimulation, respectively, used to induce fatigue. In the constant-duration experiments, larger reductions in SR function occurred following the highest frequency stimulation protocol. These data suggest that when muscles are fatigued to similar extents, depressions in SR function are independent of the activity protocol. On the other hand, when a constant duration of activity is imposed, changes in SR function are closely linked to the extent of force reduction.
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