Manufacture of Multilayered Artificial Cell Membranes through Sequential Bilayer Deposition on Emulsion Templates.
Manufacture of Multilayered Artificial Cell Membranes through Sequential Bilayer Deposition on Emulsion Templates.
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DOI：
10.1002/cbic.202100072
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发表时间：
2021-07-01
期刊：
影响因子：
--
通讯作者：
Elani Y
中科院分区：
文献类型：
--
作者：
Ip T;Li Q;Brooks N;Elani Y
制造复制生物细胞的结构、过程和行为的人工细胞的努力正在迅速增加。也许最常见的重建细胞结构是膜，通过使用单层囊泡作为模型。然而，许多细胞膜，包括细菌双膜、核膜和细胞器膜，是多层的。由于缺乏可用于其受控构建的技术，多层膜不是自下而上合成生物学中使用的细胞模拟基序的一部分。为了解决这个问题，我们开发了基于乳液的技术，允许逐层产生细胞大小的多层囊泡，并对每一层进行成分控制，从而使研究能够以其他方式进行。我们发现，弯曲刚度与层数成比例，并证明了共存的液-液域之间的双层间配准。这些技术将有助于多层膜结构的开发，为整合跨越多个双层的蛋白质复合物铺平道路。围绕囊泡运行环：我们描述了一种基于液滴的技术来制造细胞大小的多层脂质囊泡。单个膜是逐层组装的，可以控制膜层的数量及其分子含量。我们研究了结构的生物力学特性如何随着每一层的增加而变化。
Efforts to manufacture artificial cells that replicate the architectures, processes and behaviours of biological cells are rapidly increasing. Perhaps the most commonly reconstructed cellular structure is the membrane, through the use of unilamellar vesicles as models. However, many cellular membranes, including bacterial double membranes, nuclear envelopes, and organelle membranes, are multilamellar. Due to a lack of technologies available for their controlled construction, multilayered membranes are not part of the repertoire of cell‐mimetic motifs used in bottom‐up synthetic biology. To address this, we developed emulsion‐based technologies that allow cell‐sized multilayered vesicles to be produced layer‐by‐layer, with compositional control over each layer, thus enabling studies that would otherwise remain inaccessible. We discovered that bending rigidities scale with the number of layers and demonstrate inter‐bilayer registration between coexisting liquid–liquid domains. These technologies will contribute to the exploitation of multilayered membrane structures, paving the way for incorporating protein complexes that span multiple bilayers. Running rings around vesicles: We describe a droplet‐based technology to manufacture cell‐sized multilamellar lipid vesicles. Individual membranes are assembled layer‐by‐layer, giving control over the number of induvial layers and their molecular content. We investigated how the biomechanical properties of the structures change with each added layer.
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影响因子：
3.7
作者：
Green ER;Mecsas J
通讯作者：
Mecsas J
DOI：
10.1073/pnas.1620843114
发表时间：
2017-04-18
影响因子：
11.1
作者：
Koch, Marion;Wright, Katherine E.;Baum, Jake
通讯作者：
Baum, Jake
影响因子：
6.1
作者：
Kim, Shin-Hyun;Nam, Jin;Weitz, David A.
通讯作者：
Weitz, David A.
DOI：
10.1051/jphys:019900051010094500
发表时间：
1990-05-15
期刊：
JOURNAL DE PHYSIQUE
影响因子：
--
作者：
DUWE, HP;KAES, J;SACKMANN, E
通讯作者：
SACKMANN, E
影响因子：
--
作者：
Charalambous, Kalypso;Miller, David;Booth, Paula J.
通讯作者：
Booth, Paula J.