Antimicrobial Susceptibility Testing in Pseudomonas aeruginosa Biofilms: One Step Closer to a Standardized Method.
Antimicrobial Susceptibility Testing in Pseudomonas aeruginosa Biofilms: One Step Closer to a Standardized Method.
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铜绿假单胞菌生物膜的抗菌药物敏感性测试：更接近标准化方法。
DOI：
10.3390/antibiotics9120880
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发表时间：
2020-12-09
期刊：
影响因子：
--
通讯作者：
Sáenz Y
中科院分区：
文献类型：
--
作者：
Lozano C;López M;Rojo-Bezares B;Sáenz Y
铜绿假单胞菌在长期感染期间形成生物膜的能力使正确治疗患者变得困难。目前临床上的药敏试验方法都是基于对浮游细菌的研究。常规实验室需要一种标准化的方法来分析生物膜中的抗菌素敏感性。本研究的目的是建立一种简单的生物被膜模型，并研究铜绿假单胞菌在生物被膜生长过程中的药敏情况。采用微量平板法，以铜绿假单胞菌PAO1为参比菌株，对不同人工痰培养基及好氧和微需氧条件进行分析。测定了临床和非临床分离铜绿假单胞菌对头孢吡肟、亚胺培南、阿奇霉素、庆大霉素、妥布霉素和环丙沙星的药敏情况。人工合成的囊性纤维化培养液是一种较好的培养液。除阿奇霉素外，生物膜极大地提高了对受试抗菌药的耐药性。部分菌株对头孢吡肟和亚胺培南的抗生物被膜作用较差，而对妥布霉素、庆大霉素和环丙沙星的抗生物膜作用较好。在好氧条件下，阿奇霉素在生物膜中表现出比浮游状态下更好的活性。这项研究建立了有用的信息来测试铜绿假单胞菌生物被膜的抗菌药敏感性，并包括了治疗长期感染患者的可能的抗菌药选择。
The ability of Pseudomonas aeruginosa to form biofilm during a long-term infection makes it difficult to treat patients correctly. The current clinical antimicrobial susceptibility testing methods are based on the study of planktonic strains. A standardized protocol to analyze the antimicrobial susceptibility in biofilms is necessary for routine laboratories. The aims of this study were to develop a simple biofilm model and to study the antimicrobial susceptibility of P. aeruginosa strains in biofilm growth. Different artificial sputum media, and aerobiosis and microaerobiosis conditions were analyzed using a microtiter plate method and P. aeruginosa PAO1 as reference strain. Planktonic and biofilm antimicrobial susceptibility to cefepime, imipenem, azithromycin, gentamicin, tobramycin, and ciprofloxacin were determined in clinical and non-clinical P. aeruginosa strains. The Synthetic Cystic Fibrosis Medium was proposed as a good medium. The biofilm greatly increased the resistance to tested antimicrobials, except for azithromycin. Cefepime and imipenem showed poor anti-biofilm effect while tobramycin, gentamicin, and ciprofloxacin showed good activity in some strains. Azithromycin showed a better activity in biofilm than in planktonic state when aerobic conditions were used. This study establishes useful information to test antimicrobial susceptibility in P. aeruginosa biofilms, and includes possible antimicrobial options to treat long-term infected patients.
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影响因子：
3.7
作者：
Lozano C;Azcona-Gutiérrez JM;Van Bambeke F;Sáenz Y
通讯作者：
Sáenz Y
影响因子：
5.2
作者：
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通讯作者：
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影响因子：
4.9
作者：
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通讯作者：
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影响因子：
4.9
作者：
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通讯作者：
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