Great phenotypic and genetic variation among successive chronic Pseudomonas aeruginosa from a cystic fibrosis patient.
Great phenotypic and genetic variation among successive chronic Pseudomonas aeruginosa from a cystic fibrosis patient.
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DOI：
10.1371/journal.pone.0204167
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Sáenz Y
中科院分区：
文献类型：
--
作者：
Lozano C;Azcona-Gutiérrez JM;Van Bambeke F;Sáenz Y
在慢性感染期间发现不同适应的铜绿假单胞菌形态型。在这项工作中，分析了从囊性纤维化（CF）患者中连续分离的铜绿假单胞菌的相关生物学决定因素，以深入了解铜绿假单胞菌在慢性感染过程中的异质性。包括3年内从患者中收集的17株铜绿假单胞菌分离株，5株小菌落变体（SCV）和12株粘液样。进行了以下分析：脉冲场凝胶电泳（PFGE）/多位点序列分型（MLST）/血清型、抗菌药物敏感性、生长曲线、形成生物膜的能力、色素产生、弹性蛋白酶活性、运动性;毒力/群体感应基因的存在/表达，以及耐药机制的鉴定。所有分离物的PFGE图谱均为ST 412。SCV的表型和基因型存在显著差异，SCV对抗生素的耐药性高于粘液型分离株。检测AmpC过度产生和外排泵活性。7株含有2个整合子，9株仅含有1个整合子。所有SCV显示相同的OprD谱，而在粘液中鉴定出三种不同的谱。MutL和MutS没有发现氨基酸变化。所有分离株生长缓慢，通常产生高生物膜，降低了毒素表达和群体感应，并显示出低运动性。然而，SCV和粘液分离株之间存在统计学显著差异。SCV生长更快，呈现出更高的生物膜形成和flicA表达，但产生较少的绿脓菌素和绿脓菌素，显示出较低的弹性蛋白酶活性和rhlR，algD，lasB的表达比粘液样分离株。这些结果有助于了解慢性铜绿假单胞菌分离株在CF患者中的分子行为。
Different adapted Pseudomonas aeruginosa morphotypes are found during chronic infections. Relevant biological determinants in P. aeruginosa successively isolated from a cystic fibrosis (CF) patient were analyzed in this work to gain insight into P. aeruginosa heterogeneity during chronic infection. Seventeen P. aeruginosa isolates collected from a patient over a 3 year period were included, 5 small colony variants (SCV) and 12 mucoids. The following analyses were performed: Pulsed-Field-Gel-Electrophoresis (PFGE)/Multilocus- sequence-typing (MLST)/serotype, antimicrobial susceptibility, growth curves, capacity to form biofilm, pigment production, elastase activity, motility; presence/expression of virulence/quorum sensing genes, and identification of resistance mechanisms. All isolates had closely related PFGE patterns and belonged to ST412. Important phenotypic and genotypic differences were found. SCVs were more resistant to antimicrobials than mucoid isolates. AmpC hyperproduction and efflux pump activity were detected. Seven isolates contained two integrons and nine isolates only one integron. All SCVs showed the same OprD profile, while three different profiles were identified among mucoids. No amino acid changes were found in MutL and MutS. All isolates were slow-growing, generally produced high biofilm, had reduced their toxin expression and their quorum sensing, and showed low motility. Nevertheless, statistically significant differences were found among SCV and mucoid isolates. SCVs grew faster, presented higher biofilm formation and flicA expression; but produced less pyorubin and pyocyanin, showed lower elastase activity and rhlR, algD, and lasB expression than mucoid isolates. These results help to understand the molecular behavior of chronic P. aeruginosa isolates in CF patients.
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影响因子：
3
作者：
Heras J;Domínguez C;Mata E;Pascual V;Lozano C;Torres C;Zarazaga M
通讯作者：
Zarazaga M
影响因子：
4.5
作者：
Feliziani S;Marvig RL;Luján AM;Moyano AJ;Di Rienzo JA;Krogh Johansen H;Molin S;Smania AM
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影响因子：
3.7
作者：
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通讯作者：
Burrows LL
影响因子：
3
作者：
Fothergill, Joanne L.;Mowat, Eilidh;Winstanley, Craig
通讯作者：
Winstanley, Craig
影响因子：
2.8
作者：
Lee, Baoleri;Schjerling, Charlotte K.;Ciofu, Oana
通讯作者：
Ciofu, Oana