Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells.
Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells.
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DOI：
10.1186/ar1827
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发表时间：
2005
影响因子：
4.9
通讯作者：
Noël D
中科院分区：
文献类型：
--
作者：
Djouad F;Bony C;Häupl T;Uzé G;Lahlou N;Louis-Plence P;Apparailly F;Canovas F;Rème T;Sany J;Jorgensen C;Noël D
先前的研究报道，间充质干细胞（MSC）可以通过与培养滑膜成纤维细胞相同的方法从滑膜中分离出来，这表明MSC与粘附细胞群的一个子集相对应，就像骨髓间质室（BM）中的MSC一样。本研究的目的是，首先，更好地表征来自滑膜的间充质干细胞，其次，通过定量分析，系统地、平行地比较从BM中分离出的含有间充质干细胞的细胞群和来自滑膜的细胞群。荧光活化细胞分选分析显示，这两个群体的CD14、CD34和CD45表达均为阴性，CD44、CD73、CD90和CD105的表达水平相同，这是目前已知的BM-MSC的特征表型。与BM-MSC相比，这种从滑膜分离的msc样细胞首次在混合淋巴细胞反应中抑制t细胞反应，并表达与BM-MSC相同程度的吲哚胺2,3-双加氧酶活性，这可能是这种抑制活性的介质。通过定量RT-PCR，这些数据表明，来自滑膜和骨髓的间充质干细胞样细胞可能被诱导成软骨分化，在较小程度上也可能被诱导成骨分化，但基于所测试的标志物（II型胶原和聚集蛋白或碱性磷酸酶和骨钙素）的表达，滑膜来源的间充质干细胞的成骨能力显著降低。利用Atlas人细胞因子/受体阵列测定的转录谱显示，来自滑膜的msc样细胞和BM-MSC之间存在268个基因中的46个差异。特别是，激活素A被证明是一个主要的上调因子，由BM-MSC高度分泌。这是否反映了不同的细胞表型，与BM-MSC贴壁细胞群体相比，滑膜衍生群体中MSC的数量不同，或者不同微环境的影响仍有待确定。总之，尽管脑脊髓瘤来源和滑膜来源的MSC具有相似的表型和功能特性，但它们的分化能力和转录谱允许人们根据其组织来源区分细胞群。
Previous studies have reported that mesenchymal stem cells (MSC) may be isolated from the synovial membrane by the same protocol as that used for synovial fibroblast cultivation, suggesting that MSC correspond to a subset of the adherent cell population, as MSC from the stromal compartment of the bone marrow (BM). The aims of the present study were, first, to better characterize the MSC derived from the synovial membrane and, second, to compare systematically, in parallel, the MSC-containing cell populations isolated from BM and those derived from the synovium, using quantitative assays. Fluorescent-activated cell sorting analysis revealed that both populations were negative for CD14, CD34 and CD45 expression and that both displayed equal levels of CD44, CD73, CD90 and CD105, a phenotype currently known to be characteristic of BM-MSC. Comparable with BM-MSC, such MSC-like cells isolated from the synovial membrane were shown for the first time to suppress the T-cell response in a mixed lymphocyte reaction, and to express the enzyme indoleamine 2,3-dioxygenase activity to the same extent as BM-MSC, which is a possible mediator of this suppressive activity. Using quantitative RT-PCR these data show that MSC-like cells from the synovium and BM may be induced to chondrogenic differentiation and, to a lesser extent, to osteogenic differentiation, but the osteogenic capacities of the synovium-derived MSC were significantly reduced based on the expression of the markers tested (collagen type II and aggrecan or alkaline phosphatase and osteocalcin, respectively). Transcription profiles, determined with the Atlas Human Cytokine/Receptor Array, revealed discrimination between the MSC-like cells from the synovial membrane and the BM-MSC by 46 of 268 genes. In particular, activin A was shown to be one major upregulated factor, highly secreted by BM-MSC. Whether this reflects a different cellular phenotype, a different amount of MSC in the synovium-derived population compared with BM-MSC adherent cell populations or the impact of a different microenvironment remains to be determined. In conclusion, although the BM-derived and synovium-derived MSC shared similar phenotypic and functional properties, both their differentiation capacities and transcriptional profiles permit one to discriminate the cell populations according to their tissue origin.
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影响因子：
20.3
作者：
Meisel, R;Zibert, A;Dilloo, D
通讯作者：
Dilloo, D
影响因子：
20.3
作者：
Lee, OK;Kuo, TK;Chen, TH
通讯作者：
Chen, TH
影响因子：
--
作者：
Kasperkovitz, PV;Timmer, TCG;Verweij, CL
通讯作者：
Verweij, CL
影响因子：
--
作者：
Marinova-Mutafchieva, L;Williams, RO;Zvaifler, NJ
通讯作者：
Zvaifler, NJ
影响因子：
4.9
作者：
Barnes, E;Salio, M;Klenerman, P
通讯作者：
Klenerman, P