Male mice express spermatogenic cell-specific triosephosphate isomerase isozymes.
Male mice express spermatogenic cell-specific triosephosphate isomerase isozymes.
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DOI：
10.1002/mrd.22217
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发表时间：
2013-10
影响因子：
2.5
通讯作者：
Gerton, George L.
中科院分区：
文献类型：
--
作者：
Ijiri, Takashi W.;Vadnais, Melissa L.;Lin, Angel M.;Huang, Andy P.;Cao, Wenlei;Merdiushev, Tanya;Gerton, George L.
磷酸丙糖异构酶1（TPI 1）是糖酵解途径的成员，是小鼠精子运动的关键能量来源。通过免疫印迹，我们检测到两个男性，生殖系特异性TPI 1带（先生33，400和30，800）以及体细胞型带（先生27，700）。虽然这三条带都在生精细胞中观察到，但在附睾成熟过程中，体细胞型TPI 1从精子中消失。体外去磷酸化分析表明，两个男性，生殖系特异性TPI 1带不是27，700 Mr TPI 1带磷酸化的结果。Mr 33，400、30，800和27，700的Tpi 1条带分别对应于预测使用Tpi 1 cDNA的第一、第二和第三可能起始密码子的蛋白质的大小。我们对附睾精子进行了免疫荧光检测，并确定TPI 1特异性地定位于主段。附睾尾部精子的抗体染色强于附睾头部精子，这一发现与TPI 1作为附睾尾部精子富集蛋白的鉴定一致。十二烷基硫酸钠（SDS）不溶性鞭毛辅助结构的免疫荧光显示了强烈的TPI 1信号仅在主片，表明TPI 1是纤维鞘的一个组成部分。北方印迹杂交检测到较长的Tpi 1转录本（1.56 kb），在小鼠睾丸，而体细胞组织有较短的转录本（1.32 kb）。由于小鼠基因组中只有一个磷酸丙糖异构酶基因，我们得出结论，我们在精子中看到的三种变体是由生精细胞中使用替代翻译起始密码子引起的。摩尔Reprod. Dev. 80：862-870，2013.© 2013作者。由威利期刊公司出版这是一个开放获取的条款下的知识共享署名-非商业性-非衍生许可证，允许使用和分发在任何媒体，只要原作品是正确引用，使用是非商业性的，没有修改或改编。
Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed in spermatogenic cells, somatic-type TPI1 disappeared from sperm during epididymal maturation. In vitro dephosphorylation analysis suggested that the two male, germ line-specific TPI1 bands were not the result of phosphorylation of the 27,700 Mr TPI1 band. The Mr 33,400; 30,800; and 27,700 TPI1 bands corresponded to the respective sizes of the proteins predicted to use the first, second, and third possible initiation codons of the Tpi1 cDNA. We performed immunofluorescence on epididymal sperm and determined that TPI1 specifically localized in the principal piece. The antibody staining was stronger in cauda epididymal sperm than in caput epididymal sperm, a finding consistent with the identification of TPI1 as a cauda epididymal sperm-enriched protein. Immunofluorescence with sodium dodecyl sulfate (SDS)-insoluble flagellar accessory structures showed a strong TPI1 signal only in the principal piece, indicating that TPI1 is a component of the fibrous sheath. Northern blot hybridization detected longer Tpi1 transcripts (1.56 kb) in mouse testis, whereas somatic tissues had shorter transcripts (1.32 kb). As there is only one triosephosphate isomerase gene in the mouse genome, we conclude that the three variants we see in sperm result from the use of alternative translation start codons in spermatogenic cells. Mol. Reprod. Dev. 80: 862–870, 2013. © 2013 The Authors. Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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影响因子：
--
作者：
Cao, Wenlei;Ijiri, Takashi W.;Gerton, George L.
通讯作者：
Gerton, George L.
影响因子：
2.7
作者：
Johnson, LR;Foster, JA;Gerton, GL
通讯作者：
Gerton, GL
DOI：
10.1073/pnas.0407708101
发表时间：
2004-11-23
影响因子：
11.1
作者：
Miki, K;Qu, WD;O'Brien, DA
通讯作者：
O'Brien, DA
影响因子：
64.8
作者：
MCCARREY, JR;THOMAS, K
通讯作者：
THOMAS, K
影响因子：
3.6
作者：
NAKAMURA, M;OKINAGA, S;ARAI, K
通讯作者：
ARAI, K