Expression and changing distribution of the human transferrin receptor in developing Drosophila oocytes and embryos.
Expression and changing distribution of the human transferrin receptor in developing Drosophila oocytes and embryos.
复制标题
人转铁蛋白受体在发育中的果蝇卵母细胞和胚胎中的表达和分布变化。
作者：
M. Bretscher
为了更好地了解发育中的果蝇卵母细胞的膜系统，人转铁蛋白受体已经在那里表达。这是使用与K10或oskar trailer序列组合的Armadillo启动子实现的;这些使信使RNA能够在保育细胞中转录，然后运输到卵母细胞并在卵母细胞中翻译。这是第一个在卵母细胞中表达的外源蛋白。在第8阶段，转铁蛋白受体主要集中在卵母细胞的后极，并与大的细胞质囊泡;当与shibire突变相结合时，转铁蛋白受体被转移到卵膜，表明它们参与了内吞循环。在第10阶段，转铁蛋白受体定位于卵母细胞的前缘或后极，这取决于mRNA的位置。在新产的卵中，所有的转铁蛋白受体都存在于大的细胞质囊泡中。结果显示，显着的分选过程中发生的卵母细胞成熟，并显示，环管，分离的卵膜从护士细胞质膜，作为屏障，以防止在这两个隔间的成分相互混合。
In order to understand better the membrane systems in a developing Drosophila oocyte, the human transferrin receptor has been expressed there. This was achieved using the armadillo promoter combined with K10 or oskar trailer sequences; these enable the messenger RNA to be transcribed in nurse cells and then transported to, and translated in, oocytes. This is the first exogenous protein to be expressed in oocytes. At stage 8, the transferrin receptors are mainly concentrated towards the posterior pole of the oocyte and are associated with large cytoplasmic vesicles; when combined with the shibire mutation the transferrin receptors are transferred to the oolemma, demonstrating that they participate in an endocytic cycle. At stage 10, the transferrin receptors are localised either to the anterior margin of the oocyte or to the posterior pole, depending on where the mRNA is located. In newly laid eggs, all the transferrin receptors are found in large cytoplasmic vesicles. The results reveal remarkable sorting processes which occur as oocytes mature and show that ring canals, which separate the oolemma from nurse cell plasma membranes, act as barriers to prevent components in these two compartments from intermixing.
用于分析果蝇遗传嵌合体的细胞自主、普遍存在的标记。
影响因子：
2.7
作者：
Vincent,JP;Girdham,CH;O'Farrell,PH
通讯作者：
O'Farrell,PH
上皮细胞和神经元细胞的极性。
DOI：
10.1146/annurev.cb.08.110192.002143
发表时间：
1992
期刊：
Annual review of cell biology
影响因子：
--
作者：
Rodriguez-Boulan,E;Powell,SK
通讯作者：
Powell,SK
影响因子：
10.5
作者：
RIGGLEMAN, B;WIESCHAUS, E;SCHEDL, P
通讯作者：
SCHEDL, P