The increased but non-predominant expression of Th17- and Th1-specific cytokines in Hashimoto's thyroiditis but not in Graves' disease.
The increased but non-predominant expression of Th17- and Th1-specific cytokines in Hashimoto's thyroiditis but not in Graves' disease.
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DOI：
10.1590/s0100-879x2012007500168
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Zhang JA
中科院分区：
文献类型：
--
作者：
Qin Q;Liu P;Liu L;Wang R;Yan N;Yang J;Wang X;Pandey M;Zhang JA
桥本甲状腺炎(HT)被认为主要由Th1细胞介导，但目前尚不清楚Graves病(GD)是否与Th1或Th2优势有关。Th17细胞是一种新的Th细胞亚群，在多种自身免疫性疾病的发病机制中起着至关重要的作用。本研究探讨了IL-17A和干扰素-γ在甲状旁腺功能亢进和甲亢患者中的表达。应用实时荧光定量聚合酶链式反应技术检测43例自身免疫性甲状腺疾病患者外周血单个核细胞和40例自身免疫性甲状腺疾病患者甲状腺组织中IL-17A和干扰素-γ的表达。采用免疫组织化学方法检测28例AITD患者甲状腺组织中IL-17A和IL-23p19的蛋白表达。甲亢患者外周血单个核细胞和甲状腺组织中IL-17A和干扰素-γ的表达水平均高于对照组(以细胞因子/β-肌动蛋白比值计算，IL-17分别为13.58倍和2.88倍，干扰素-γ分别为16.54倍和2.74倍，P均<0.05)。GD患者IL-17A和干扰素-γ的表达水平差异无统计学意义(P>；0.05)。免疫组织化学结果显示，IL-17A(IOD=15.17±4.8)和IL-23p19(IOD=16.84±7.87)在甲状旁腺高表达(P<0.05)。IL-17A和干扰素-γ的类似高水平提示，在甲状旁腺疾病中存在Th17和Th1的混合反应，这两种细胞可能在细胞介导的细胞毒作用的破坏过程中发挥重要作用。
Hashimoto's thyroiditis (HT) is considered to be mediated mainly by Th1 cells, but it is not known whether Graves' disease (GD) is associated with Th1 or Th2 predominance. Th17 cells, a novel subset of Th cells, play a crucial role in the pathogenesis of various autoimmune disorders. In the present study, the expression of IL-17A and IFN-γ was investigated in patients with HT or GD. mRNA expression of IL-17A and IFN-γ in peripheral blood mononuclear cells (PBMC) from 43 patients with autoimmune thyroid disease (AITD) and in thyroid tissues from 40 AITD patients were measured by real-time quantitative PCR. The protein expression of IL-17A and IL-23p19 was examined by immunohistochemistry in thyroid tissues from 28 AITD patients. The mRNA levels of IL-17A and IFN-γ were higher in both PBMC and thyroid tissues of HT patients than in controls (mRNA levels are reported as the cytokine/β-actin ratio: IL-17 = 13.58- and 2.88-fold change and IFN-γ = 16.54- and 2.74-fold change, respectively, P < 0.05). Also, the mRNA levels of IL-17A and IFN-γ did not differ significantly in GD patients (P > 0.05). The high protein expression of IL-17A (IOD = 15.17 ± 4.8) and IL-23p19 (IOD = 16.84 ± 7.87) in HT was confirmed by immunohistochemistry (P < 0.05). The similar high levels of IL-17A and IFN-γ suggest a mixed response of Th17 and Th1 in HT, where both cells may play important roles in the destruction procedure by cell-mediated cytotoxicity.
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