Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development.
Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development.
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锌缺乏和砷暴露对斑马鱼（Danio Rerio）发育的组合作用。
DOI：
10.1371/journal.pone.0183831
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Ho E
中科院分区：
文献类型：
--
作者：
Beaver LM;Truong L;Barton CL;Chase TT;Gonnerman GD;Wong CP;Tanguay RL;Ho E
缺锌和长期低水平接触饮用水中的无机砷都是影响包括孕妇在内的数百万人的重大公共卫生问题。这两种情况可以在人群中共存，但对它们的相互作用知之甚少，特别是缺锌是否会使个体对砷暴露和毒性敏感，特别是在发育的关键窗口期间。为了解决这一问题，我们利用斑马鱼模型来检验这一假设，即父母缺锌使发育中的胚胎对低浓度砷毒性敏感，从而改变发育结果。成年斑马鱼喂养规定的缺锌和锌充足的饮食，并产生锌充足和缺锌胚胎。胚胎用环境相关浓度0，50和500 ppb砷处理。砷暴露显着减少锌的量在发育中的胚胎约7%。锌缺乏和低水平砷暴露的组合并没有使发育中的胚胎对发育畸形或死亡率的增加敏感。这种组合确实导致胚胎的身体活动下降了40%，并且这种下降明显大于单独缺锌或砷暴露的观察结果。在锌缺乏或锌缺乏和砷暴露的胚胎中发现了调节锌稳态、氧化应激反应和胰岛素产生的基因（包括zip1、znt7、nrf2、ogg1、pax4和insa）的RNA表达的显著变化。总的来说，数据表明，缺锌和砷暴露的结合对发育中的胚胎产生有害影响，并可能增加患糖尿病等慢性疾病的风险。
Zinc deficiency and chronic low level exposures to inorganic arsenic in drinking water are both significant public health concerns that affect millions of people including pregnant women. These two conditions can co-exist in the human population but little is known about their interaction, and in particular, whether zinc deficiency sensitizes individuals to arsenic exposure and toxicity, especially during critical windows of development. To address this, we utilized the Danio rerio (zebrafish) model to test the hypothesis that parental zinc deficiency sensitizes the developing embryo to low-concentration arsenic toxicity, leading to altered developmental outcomes. Adult zebrafish were fed defined zinc deficient and zinc adequate diets and were spawned resulting in zinc adequate and zinc deficient embryos. The embryos were treated with environmentally relevant concentrations of 0, 50, and 500 ppb arsenic. Arsenic exposure significantly reduced the amount of zinc in the developing embryo by ~7%. The combination of zinc deficiency and low-level arsenic exposures did not sensitize the developing embryo to increased developmental malformations or mortality. The combination did cause a 40% decline in physical activity of the embryos, and this decline was significantly greater than what was observed with zinc deficiency or arsenic exposure alone. Significant changes in RNA expression of genes that regulate zinc homeostasis, response to oxidative stress and insulin production (including zip1, znt7, nrf2, ogg1, pax4, and insa) were found in zinc deficient, or zinc deficiency and arsenic exposed embryos. Overall, the data suggests that the combination of zinc deficiency and arsenic exposure has harmful effects on the developing embryo and may increase the risk for developing chronic diseases like diabetes.
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DOI：
10.1016/j.cbpc.2011.05.002
发表时间：
2012-01
影响因子：
3.9
作者：
Ho, Emily;Dukovcic, Stephanie;Hobson, Brad;Wong, Carmen P.;Miller, Galen;Hardin, Karin;Traber, Maret G.;Tanguay, Robert L.
通讯作者：
Tanguay, Robert L.
影响因子：
3.9
作者：
Franklin, RB;Ma, J;Costello, LC
通讯作者：
Costello, LC
DOI：
10.1002/tera.1420510207
发表时间：
1995-02-01
期刊：
TERATOLOGY
影响因子：
--
作者：
JANKOWSKI, MA;URIUHARE, JY;KEEN, CL
通讯作者：
KEEN, CL
影响因子：
3
作者：
Jurowski, Kamil;Szewczyk, Bernadeta;Nowak, Gabriel;Piekoszewski, Wojciech
通讯作者：
Piekoszewski, Wojciech
影响因子：
4.8
作者：
Ding, Wei;Liu, Wenlan;Liu, Ke Jian
通讯作者：
Liu, Ke Jian