Zinc transporter expression in zebrafish (Danio rerio) during development.
Zinc transporter expression in zebrafish (Danio rerio) during development.
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DOI：
10.1016/j.cbpc.2011.05.002
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发表时间：
2012-01
影响因子：
3.9
通讯作者：
Tanguay, Robert L.
中科院分区：
文献类型：
--
作者：
Ho, Emily;Dukovcic, Stephanie;Hobson, Brad;Wong, Carmen P.;Miller, Galen;Hardin, Karin;Traber, Maret G.;Tanguay, Robert L.
锌是一种微量营养素，在包括生长和发育在内的几个生物过程中非常重要。由于缺乏体内实验模型，使我们能够直接研究母体锌对植入后胚胎发育的影响，我们对母体锌缺乏对发育中胚胎的锌和锌调节机制的影响的了解有限。为了克服这一障碍，我们提议使用斑马鱼作为模式生物来研究锌在发育过程中的影响。本研究的目的是分析斑马鱼胚胎和幼体发育过程中所有已知锌转运蛋白基因的mRNA表达，并定量这些相应发育时间点的胚胎锌浓度。在受精后0、2、6、12、24、48和120 h对斑马鱼胚胎中的SLC 30 A锌转运蛋白家族（ZnT）和SCL 39 A家族、Zir-、Irt-like蛋白（ZIP）锌转运蛋白进行分析，以捕获从单细胞到完全发育的表达模式。我们观察到一致的胚胎锌水平，但在整个发展的几个锌转运蛋白的差异表达。这些结果表明，斑马鱼是一种有效的模式生物，以研究锌缺乏的影响和进一步的调查正在进行中，以确定可能的分子途径，与母亲锌缺乏失调。
Zinc is a micronutrient important in several biological processes including growth and development. We have limited knowledge on the impact of maternal zinc deficiency on zinc and zinc regulatory mechanisms in the developing embryo due to a lack of in vivo experimental models that allow us to directly study the effects of maternal zinc on embryonic development following implantation. To overcome this barrier, we have proposed to use zebrafish as a model organism to study the impact of zinc during development. The goal of the current study was to profile the mRNA expression of all the known zinc transporter genes in the zebrafish across embryonic and larval development and to quantify the embryonic zinc concentrations at these corresponding developmental time points. The SLC30A zinc transporter family (ZnT) and SCL39A family, Zir-,Irt-like protein (ZIP) zinc transporter proteins were profiled in zebrafish embryos at 0, 2, 6, 12, 24, 48 and 120 hours post fertilization to capture expression patterns from a single cell through full development. We observed consistent embryonic zinc levels, but differential expression of several zinc transporters across development. These results suggest that zebrafish is an effective model organism to study the effects of zinc deficiency and further investigation is underway to identify possible molecular pathways that are dysregulated with maternal zinc deficiency.
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DOI：
10.1073/pnas.0709307104
发表时间：
2007-11-20
影响因子：
11.1
作者：
Li, Min;Zhang, Yuqing;Yao, Qizhi
通讯作者：
Yao, Qizhi
DOI：
10.1002/tera.1420510207
发表时间：
1995-02-01
期刊：
TERATOLOGY
影响因子：
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作者：
JANKOWSKI, MA;URIUHARE, JY;KEEN, CL
通讯作者：
KEEN, CL
影响因子：
3.7
作者：
Fu Y;Tian W;Pratt EB;Dirling LB;Shyng SL;Meshul CK;Cohen DM
通讯作者：
Cohen DM
影响因子：
14.8
作者：
Kim, Alison M.;Vogt, Stefan;O'Halloran, Thomas V.;Woodruff, Teresa K.
通讯作者：
Woodruff, Teresa K.
DOI：
10.1006/bbrc.2002.6517
发表时间：
2002-03-08
影响因子：
3.1
作者：
Chen, WY;John, JAC;Chang, CY
通讯作者：
Chang, CY