Systematic analysis of CCCH zinc finger family in Brassica napus showed that BnRR-TZFs are involved in stress resistance.
Systematic analysis of CCCH zinc finger family in Brassica napus showed that BnRR-TZFs are involved in stress resistance.
复制标题
DOI：
10.1186/s12870-021-03340-8
复制
发表时间：
2021-11-23
影响因子：
5.3
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Pi B;Pan J;Xiao M;Hu X;Zhang L;Chen M;Liu B;Ruan Y;Huang Y
CCCH锌指蛋白家族是与多种生物和非生物胁迫相关的最大的转录因子家族之一。甘蓝型油菜，一种异源四倍体油料作物，由两种二倍体祖先芜菁和甘蓝自然杂交形成。油菜CCCH家族基因的系统鉴定缺失，其功能鉴定仍处于起步阶段。本研究共获得155个CCCH基因，其中81个来自其亲本B。rapa和74来自B。在B中鉴定出15个亚科。油菜。组织和共线分析解释了CCCH基因在B中的分布和共线性关系，以及重复基因对的选择压力和进化。油菜基因组在B中发现44对二倍体重复基因和4个三倍体重复基因组。在B中，CCCH基因的片段重复是由大部分CCCH基因的重复事件引起的。油菜。在B中存在9种类型的CCCH基序。其中C-X7/8-C-X5-C-X3-H基序是最常见的，并鉴定了一个新的CCH基序（C-X5-C-X3-H）。此外，在27个IX亚家族（RR-TZF）基因的启动子中存在丰富的胁迫相关顺式元件，其表达谱表明RR-TZF基因可能参与了植物对激素和非生物胁迫的应答。本研究结果为了解B中CCCH基因家族的基本特征和基因进化提供了基础。为油菜科作物的抗逆基因工程提供了潜在的目标。在线版本包含补充材料，可通过10.1186/s12870-021-03340-8获得。
CCCH zinc finger family is one of the largest transcription factor families related to multiple biotic and abiotic stresses. Brassica napus L., an allotetraploid oilseed crop formed by natural hybridization between two diploid progenitors, Brassica rapa and Brassica oleracea. A systematic identification of rapeseed CCCH family genes is missing and their functional characterization is still in infancy. In this study, 155 CCCH genes, 81 from its parent B. rapa and 74 from B. oleracea, were identified and divided into 15 subfamilies in B. napus. Organization and syntenic analysis explained the distribution and collinearity relationship of CCCH genes, the selection pressure and evolution of duplication gene pairs in B. napus genome. 44 diploid duplication gene pairs and 4 triple duplication gene groups were found in B. napus of CCCH family and the segmental duplication is attributed to most CCCH gene duplication events in B. napus. Nine types of CCCH motifs exist in B. napus CCCH family members, and motif C-X7/8-C-X5-C-X3-H is the most common and a new conserved CCH motif (C-X5-C-X3-H) has been identified. In addition, abundant stress-related cis-elements exist in promoters of 27 subfamily IX (RR-TZF) genes and their expression profiles indicated that RR-TZF genes could be involved in responses to hormone and abiotic stress. The results provided a foundation to understand the basic characterization and genes evolution of CCCH gene family in B. napus, and provided potential targets for genetic engineering in Brassicaceae crops in pursuit of stress-tolerant traits. The online version contains supplementary material available at 10.1186/s12870-021-03340-8.
登录
查看更多内容
影响因子：
5.6
作者：
He, Xin;Wang, Tianyi;Zhu, Longfu
通讯作者：
Zhu, Longfu
影响因子：
16.6
作者：
Cano F;Rapiteanu R;Sebastiaan Winkler G;Lehner PJ
通讯作者：
Lehner PJ
影响因子：
4.6
作者：
Huang Y;Mo Y;Chen P;Yuan X;Meng F;Zhu S;Liu Z
通讯作者：
Liu Z
大麦 (Hordeum vulgare L.) 中 APETALA2/乙烯反应因子 (AP2/ERF) 基因家族的全基因组分析
影响因子：
3.7
作者：
Guo B;Wei Y;Xu R;Lin S;Luan H;Lv C;Zhang X;Song X;Xu R
通讯作者：
Xu R
影响因子：
5.6
作者：
D'Orso F;De Leonardis AM;Salvi S;Gadaleta A;Ruberti I;Cattivelli L;Morelli G;Mastrangelo AM
通讯作者：
Mastrangelo AM