Ultrathin Cryosections: An Important Tool for Immunofluorescence and Correlative Microscopy1
Ultrathin Cryosections: An Important Tool for Immunofluorescence and Correlative Microscopy1
复制标题
超薄冷冻切片：免疫荧光和相关显微镜的重要工具1
DOI：
10.1177/002215540305100602
复制
发表时间：
2003
影响因子：
3.2
通讯作者：
J. M. Robinson
中科院分区：
文献类型：
--
作者：
T. Takizawa;J. M. Robinson
在这里，我们证明胎盘组织的超薄冷冻切片可以用作免疫荧光实验的基质。这些制剂可以实现高度的空间分辨率，因为基本上不存在离焦荧光。因此，使用超薄冰冻切片的免疫荧光显微镜提供了一种非常有用的方法来确定组织中抗原的精确亚细胞定位。此外，胎盘超薄冷冻切片还可作为使用 FluoroNanogold 作为检测系统的相关免疫荧光和免疫电子显微镜的基质。在相关显微镜中，通过荧光显微镜和电子显微镜观察到同一超薄切片中完全相同的结构。使用颗粒计数程序和电子显微镜，我们比较了用胶体金和 FluoroNanogold 获得的标记，发现银增强 FluoroNanogold 的颗粒数量高于胶体金。
Here we show that ultrathin cryosections of placental tissue can be used as a substrate in immunofluorescence experiments. A high degree of spatial resolution can be achieved in these preparations because there is essentially no out-of-focus fluorescence. Therefore, immunofluorescence microscopy using ultrathin cryosections provides a very useful method for determining the precise subcellular localization of antigens in tissues. In addition, ultrathin cryosections of placenta also serve as a substrate for correlative immunofluorescence and immunoelectron microscopy using FluoroNanogold as the detection system. In correlative microscopy, the exact same structures in the same ultrathin section were observed by both fluorescence and electron microscopy. Using a particle counting procedure and electron microscopy, we compared the labeling obtained with colloidal gold and FluoroNanogold and found a higher number of particles with silver-enhanced FluoroNanogold than with colloidal gold.
影响因子：
3.8
作者：
Lyden, TW;Anderson, CL;Robinson, JM
通讯作者：
Robinson, JM
超薄冷冻切片相关显微镜是胎盘研究的有力工具。
影响因子：
3.8
作者：
Takizawa,T;Robinson,JM
通讯作者：
Robinson,JM