TBI with lung dose reduction does not improve hematopoietic cell homing to BM during allogeneic transplantation.
TBI with lung dose reduction does not improve hematopoietic cell homing to BM during allogeneic transplantation.
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DOI：
10.1038/bmt.2009.121
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发表时间：
2010-01
影响因子：
4.8
通讯作者：
Barrett, A. J.
中科院分区：
文献类型：
--
作者：
Singh, A. K.;Chen, J.;Calado, R.;Sowers, A.;Mitchell, J. B.;Barrett, A. J.
目的：探讨全身照射（TBI）剂量、分离和肺屏蔽对造血干细胞归巢的影响。从B6- gfp小鼠胫骨和股骨中提取骨髓（BM）细胞，移植到B6小鼠体内。受体小鼠有：1)无辐射，2)单剂量13.6 Gy （Gy）的TBI， 3)单剂量13.6 Gy的TBI，通过屏蔽使肺暴露量减少到0.4 Gy, 4) 12 Gy至两次/天的分剂量TBI，持续4天，或5)12 Gy至两次/天的分剂量TBI，通过屏蔽使肺暴露量减少到0.36 Gy，持续4天。最后一次照射在尾静脉注射前4-6小时。18小时后处死小鼠。与对照组相比，任何辐照组中GFP阳性、谱系阴性细胞的归巢率均无显著提高。GFP阳性、谱系阴性、Kit阳性细胞归巢率在所有辐照组均显著降低。TBI不能改善谱系阴性供体骨髓细胞向受体骨髓的归巢。谱系阴性，Kit阳性的供体BM细胞在TBI后归巢明显更差，有或没有肺剂量减少。
To determine the effects of total body irradiation (TBI) dose, fractionation, and lung shielding on hematopoietic stem cell homing to the bone marrow. Bone marrow (BM) cells were extracted from tibiae and femurs of B6-GFP mice and were transplanted into B6 mice. Recipient mice had either: 1) no radiation, 2) single dose TBI at 13.6 Gray (Gy), 3) single dose TBI at 13.6 Gy with reduced lung exposure to 0.4 Gy by shielding, 4) split dose TBI at 12 Gy to twice/day over four days, or 5) split dose TBI at 12 Gy to twice/day over four days with reduced lung exposure to 0.36 Gy by shielding. The last radiation exposure preceded tail vein injection by 4–6 hours. Mice were sacrificed after 18 hours. Homing of GFP positive, lineage negative cells was not significantly improved in any irradiated group compared to control. Homing of GFP positive, lineage negative, Kit positive cells was significantly worse in all irradiated groups. TBI does not improve the homing of lineage negative donor BM cells to the recipient marrow. Homing of lineage negative, Kit positive donor BM cells was significantly worse following TBI, with or without lung dose reduction.
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