Hemolysis of rat erythrocytes by replacement of the natural phosphatidylcholine by various phosphatidylcholines.
Hemolysis of rat erythrocytes by replacement of the natural phosphatidylcholine by various phosphatidylcholines.
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通过用各种磷脂酰胆碱替代天然磷脂酰胆碱引起大鼠红细胞溶血。
DOI：
10.1111/j.1432-1033.1980.tb04846.x
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
L. van Deenen
中科院分区：
文献类型：
--
作者：
L. G. Lange;G. van Meer;J. Op den Kamp;L. van Deenen
在特定磷脂酰胆碱交换蛋白存在的情况下，多种类型的磷脂酰胆碱的囊泡已被证明是完整大鼠红细胞的磷脂酰胆碱的合适供体。与磷脂酰胆碱交换的进展同时发生的是溶血的发生，其发生程度针对所使用的磷脂酰胆碱类型的交换特征。在与二肉豆蔻酰、二棕榈酰和二硬脂酰物质交换期间，当 27%、25% 和 22% 的天然磷脂酰胆碱分别被这些二饱和物质取代时，溶血开始。在二油酰甘油磷酸胆碱和1-硬脂酰-2-油酰甘油磷酸胆碱的情况下，分别在引入30%和28%后开始溶血。相比之下，用鸡蛋磷脂酰胆碱替代天然红细胞磷脂酰胆碱，且二油酰类物质的水平高达60%，不会导致快速溶血。尽管存在这些功能后果，但个体磷脂和胆固醇的总体含量仍正常。伴随生化事件的是形态变化，包括棘状红细胞和球形红细胞的形成。讨论了溶血的结构功能含义和可能的机制。
Vesicles of a variety of types of phosphatidylcholine have been shown to be suitable donors of phosphatidylcholine to intact rat erythrocytes in the presence of a specific phosphatidylcholine exchange protein. Coincident with the progression of phosphatidylcholine exchange is the onset of hemolysis, occurring at degrees of exchange characteristic for the type of phosphatidylcholine employed. During exchange with the dimyristoyl, dipalmitoyl and distearoyl species, hemolysis starts when 27%, 25% and 22% of the native phosphatidylcholine is replaced by these disaturated species, respectively. In the case of dielaidoylglycerophosphocholine and 1-stearoyl-2-oleoylglycerophosphocholine hemolysis starts after the introduction of 30% and 28%, respectively. In contrast, the replacement of native erythrocyte phosphatidylcholine with egg phosphatidylcholine, and the dioleoyl species up to levels of 60% does not result in rapid hemolysis. Despite such functional consequences, overall contents of the individual phospholipids and cholesterol are normal. Accompanying the biochemical events are morphologic changes, including echinocyte an spherocyte formation. The structure-function implications and possible mechanisms of hemolysis are discussed.
影响因子：
6.5
作者：
W. R. Morrison;L. Smith
通讯作者：
W. R. Morrison;L. Smith