Modulation of the differentiation of dental pulp stem cells by different concentrations of β-glycerophosphate.
Modulation of the differentiation of dental pulp stem cells by different concentrations of β-glycerophosphate.
复制标题
通过不同浓度的β-甘油磷酸盐对牙髓干细胞分化的调节。
DOI：
10.3390/molecules17021219
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发表时间：
2012-01-31
期刊：
影响因子：
--
通讯作者：
Hu W
中科院分区：
文献类型：
--
作者：
Liu M;Sun Y;Liu Y;Yuan M;Zhang Z;Hu W
牙本质发生是牙组织工程的必要先决条件。牙本质发生的步骤之一是获得大量高度纯化的成牙本质细胞。因此，我们进行了一项实验，应用不同浓度的β-磷酸甘油（β-GP）在28天的长期培养中诱导牙髓干细胞（DPSC）的分化。同时，我们研究了基质细胞外磷酸糖蛋白（MEPE）和成牙本质细胞样标记物牙本质唾液蛋白（DSP）的时间和成熟依赖性表达，以研究优化的矿化条件。 Western blot结果显示，随着β-GP浓度的增加，DSP的表达量降低，并且不同浓度的β-GP对MEPE的表达也有影响。同时，与对照组相比，矿化组对MEPE的表达具有抑制作用。最重要的是，所有实验组都成功地通过茜素红 S 生成了矿化结节，并且 5 mM β-GP 组形成了更多的矿化结节，并使用 CPC 提取方法进行了定量。总之，在 DPSC 的分化过程中，β-GP 发生了显着的调节。成牙本质细胞分化的程度依赖于β-甘油磷酸浓度。低浓度的 β-GP (5 mM) 已被证明是刺激 DPSC 成熟的最佳浓度。而且，MEPE与DSP的结合清楚地表明了差异化程度。
Dentinogenesis is a necessary prerequisite for dental tissue engineering. One of the steps for dentinogenesis is to obtain large quantities of highly purified odontoblasts. Therefore, we have undertaken an experiment applying different concentrations of β-glycerophosphate (β-GP) to induce the differentiation of dental pulp stem cells (DPSCs) in a long-term 28-day culture. In the meanwhile, we have studied the time- and maturation-dependent expression of matrix extracellular phosphoglycoprotein (MEPE) and that of the odontoblast-like marker-dentin sialoprotein (DSP), in order to investigate an optimized mineralized condition. Western blot results revealed that the expression of DSP became lower when accompanied by the increase of the β-GP concentration, and there was also an influence on MEPE expression when different concentrations of β-GP were applied. Meanwhile, the mineralized groups had an inhibitory function on the expression of MEPE as compared with the control group. Above all, all experimental groups successfully generated mineralized nodules by Alizarin Red S and the 5 mM β-GP group formed more mineralized nodules quantitated using the CPC extraction method. In conclusion, there is a significant modulation of the β-GP during the differentiation of the DPSCs. The degree of odontoblast differentiation is β-glycerophosphate concentration dependent. A low concentration of β-GP (5 mM) has been shown to be the optimal concentration for stimulating the maturation of the DPSCs. Moreover, MEPE accompanied with DSP clearly demonstrates the degree of the differentiation.
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影响因子：
4.1
作者：
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通讯作者：
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影响因子：
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