Barley lys3 mutants are unique amongst shrunken-endosperm mutants in having abnormally large embryos.

Barley lys3 mutants are unique amongst shrunken-endosperm mutants in having abnormally large embryos.
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DOI:
10.1016/j.jcs.2018.04.013
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发表时间:
2018-07
影响因子:
3.8
通讯作者:
Trafford K
Trafford K
中科院分区:
农林科学2区
文献类型:
--
作者:
Cook F;Hughes A;Nibau C;Orman-Ligeza B;Schatlowski N;Uauy C;Trafford K

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大麦(Hordeum vulgare L.)已经描述过,并且已知其中几种在淀粉生物合成基因中具有损伤。在这里,我们证实,一种类型的收缩胚乳突变体,lys 3(这么叫,因为它是第一次被确定为高赖氨酸突变体)有一个额外的表型:以及收缩胚乳,它也有扩大的胚胎。lys 3胚胎的干重比正常胚胎大50-150%。对发育中的lys 3胚的观察表明,它们经历了一种形式的过早萌发,成熟的lys 3谷粒显示出降低的休眠。在许多方面,大麦lys 3的表型与水稻巨胚突变体(OsGE基因受影响)的表型相似。然而,OsGE的大麦直向同源物位于与Lys 3不同的染色体上。这些结果表明,Lys 3基因不太可能编码淀粉生物合成蛋白,而是影响籽粒发育的蛋白质。
Many shrunken endosperm mutants of barley (Hordeum vulgare L.) have been described and several of these are known to have lesions in starch biosynthesis genes. Here we confirm that one type of shrunken endosperm mutant, lys3 (so called because it was first identified as a high-lysine mutant) has an additional phenotype: as well as shrunken endosperm it also has enlarged embryos. The lys3 embryos have a dry weight that is 50–150% larger than normal. Observations of developing lys3 embryos suggest that they undergo a form of premature germination and the mature lys3 grains show reduced dormancy. In many respects, the phenotype of barley lys3 is similar to that of rice GIANT EMBRYO mutants (affected in the OsGE gene). However, the barley orthologue of OsGE is located on a different chromosome from Lys3. Together these results suggest that the gene underlying Lys3 is unlikely to encode a starch biosynthesis protein but rather a protein influencing grain development.
DOI: 10.1186/s13007-017-0229-8
发表时间: 2017
期刊: Plant methods
影响因子: 5.1
作者:
Hughes A;Askew K;Scotson CP;Williams K;Sauze C;Corke F;Doonan JH;Nibau C
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