Serum expression levels of Mb and NT-proBNP and NF-κB expression in neutrophils in patients with MODS and the clinical significance.
Serum expression levels of Mb and NT-proBNP and NF-κB expression in neutrophils in patients with MODS and the clinical significance.
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DOI：
10.3892/etm.2018.6659
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发表时间：
2018-11
影响因子：
2.7
通讯作者：
Liu R
中科院分区：
文献类型：
--
作者：
Wang C;Chen Q;Cui Z;Xu Z;Shi Y;Su S;Qi L;Geng Y;Liu R
本研究旨在探讨多器官功能障碍综合征（MODS）患者血清中肌红蛋白（Mb）、n端前脑利钠肽（NT-proBNP）及中性粒细胞中核因子-κB （NF-κB）的表达及其临床意义。选取2014年7月至2015年12月在河南省第三人民医院（中国郑州）急诊科住院的重症患者314例。选择MODS患者70例作为MODS组，选择有单一器官或系统损伤的患者108例作为重点预防组，选择无明显器官或系统损伤的患者136例作为预防组。ELISA法检测血清Mb和NT-proBNP水平。采用流式细胞术分离中性粒细胞，电泳迁移位移法（EMSA）检测MODS患者中性粒细胞中NF-κB活性。治疗后1、7、14 d，三组患者血清Mb、NT-proBNP、NF-κB水平均较治疗前显著降低（p<0.05）。Logistic回归分析显示，血清Mb、NT-proBNP、NF-κB活性与病情进展呈正相关（r=0.809、0.785、0.833,p=0.012、0.025、0.004），与治疗时间呈负相关（r= - 0.773、- 0.734、- 0.815,p=0.033、0.041、0.078）。Mb、NT-proBNP和NF-κB可能参与了MODS的发病发展过程，并可能在MODS的预防和治疗中发挥重要作用。
This study was designed to investigate the expression of myoglobin (Mb), N-terminal pro-brain natriuretic peptide (NT-proBNP) in serum and the expression of nuclear factor-κB (NF-κB) in neutrophils in patients with multiple organ dysfunction syndrome (MODS) and the clinical significance. From July 2014 to December 2015, 314 patients with severe disease were selected during their stays at the emergency ward of the Third People's Provincial Hospital of Henan Province (Zhengzhou, China). Seventy patients with MODS were selected as MODS group, 108 patients with single organ or system injury were selected as the key prevention group, and 136 patients without obvious organ or systemic injury were selected as prevention group. Serum levels of Mb and NT-proBNP were measured by ELISA. Neutrophils were isolated by flow cytometry, and the activity of NF-κB in neutrophils of MODS patients was measured by electrophoretic mobility shift assay (EMSA). At 1, 7 and 14 days after treatment, the levels of Mb, NT-proBNP and NF-κB in the three groups were significantly reduced compared with pretreatment levels (p<0.05). Logistic regression analysis showed that serum Mb, NT-proBNP and NF-κB activity were positively correlated with the progression of the disease (r=0.809, 0.785 and 0.833, p=0.012, 0.025 and 0.004), and negatively correlated with the treatment time (r=−0.773, −0.734 and −0.815; p=0.033, 0.041 and 0.078). Mb, NT-proBNP and NF-κB may be involved in the pathogenesis and development of MODS, and may play an important role in the prevention and treatment of MODS.
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影响因子：
37.8
作者：
Bolli R;Tang XL;Sanganalmath SK;Rimoldi O;Mosna F;Abdel-Latif A;Jneid H;Rota M;Leri A;Kajstura J
通讯作者：
Kajstura J
影响因子：
4.5
作者：
Garding A;Bhattacharya N;Claus R;Ruppel M;Tschuch C;Filarsky K;Idler I;Zucknick M;Caudron-Herger M;Oakes C;Fleig V;Keklikoglou I;Allegra D;Serra L;Thakurela S;Tiwari V;Weichenhan D;Benner A;Radlwimmer B;Zentgraf H;Wiemann S;Rippe K;Plass C;Döhner H;Lichter P;Stilgenbauer S;Mertens D
通讯作者：
Mertens D
影响因子：
3.7
作者：
Szijarto, Attila;Turoczi, Zsolt;Lotz, Gabor
通讯作者：
Lotz, Gabor
影响因子：
4.1
作者：
Rajasekaran, Surender;Kruse, Katherine;Birmingham, James
通讯作者：
Birmingham, James
影响因子：
3.7
作者：
Licata, Anna;Corrao, Salvatore;Craxi, Antonio
通讯作者：
Craxi, Antonio