Identification and characterization of PyAQPs from Pyropia yezoensis, which are involved in tolerance to abiotic stress
Identification and characterization of PyAQPs from Pyropia yezoensis, which are involved in tolerance to abiotic stress
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条斑紫菜 PyAQP 的鉴定和表征，其参与非生物胁迫耐受性
DOI：
10.1007/s10811-016-1042-x
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发表时间：
2017-01
影响因子：
3.3
通讯作者：
Mao Yunxiang
中科院分区：
文献类型：
--
作者：
Kong Fanna;Yang Junqing;Li Na;Zhao Hailong;Mao Yunxiang
水通道蛋白是一类古老的通道蛋白。水通道蛋白在陆生植物中的作用已被广泛研究。然而，关于海藻中AQP的信息尚不清楚。条斑甲藻（Pyropia yezoensis）（红藻门）生长在潮间带，被认为是潮间带海藻的模式研究生物。在本研究中，我们从条斑紫菜中分离出两个水通道蛋白（PyAQP）。它们含有2个保守的Asn-Pro-Ala（NPA）基序和6个跨膜α螺旋（H1-H6）。系统发育分析表明，它们可分为两个亚科，与绿色植物的进化距离远。PyAQP 1属于Glp组，而PyAQP 2属于单个小组。亚细胞定位结果显示，PyAQP 1主要分布于细胞质膜，PyAQP 2主要分布于细胞质膜和细胞质。这两个基因在mRNA和蛋白质水平上显示出不同的非生物胁迫（干燥，盐度和温度）的差异表达谱。PyAQP在酵母细胞中的过表达表明PyAQP促进了酵母在渗透胁迫下的生长。这些结果为进一步了解条斑紫菜响应逆境胁迫的分子机制提供了理论依据。
Aquaporins are a class of ancient channel proteins. The role of aquaporin proteins (AQPs) has been extensively studied in land plants. However, the information on AQPs in seaweeds is unclear. Pyropia yezoensis (Rhodophyta) grows in the intertidal zone and is believed to be a model research organism for seaweeds in the intertidal zone. In this study, we isolated two AQPs from P. yezoensis (PyAQPs). They contained two conserved Asn-Pro-Ala (NPA) motifs and six transmembrane α-helices (H1–H6). Phylogenetic analysis showed that they are classified into two subfamilies, with much greater evolutionary distance with green plants. PyAQP1 belonged to the Glp group, whereas PyAQP2 belonged to a single small group. Subcellular localization showed that PyAQP1 was distributed on the plasma membrane of the cells, and PyAQP2 was mainly distributed in the plasma membrane and cytoplasm of the cells. The two genes displayed differential expression profiles for different abiotic stresses (desiccation, salinity, and temperature) at the mRNA and protein levels. Over-expression of PyAQPs in yeast cells indicated that PyAQPs improved the growth of yeast under osmotic stress. These results provide an insight towards understanding the molecular mechanisms of P. yezoensis responding to stress.
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影响因子：
11.4
作者：
MAUREL, C;REIZER, J;CHRISPEELS, MJ
通讯作者：
CHRISPEELS, MJ
影响因子：
7.4
作者：
Chaumont, F;Barrieu, F;Jung, R
通讯作者：
Jung, R
影响因子：
4.9
作者：
Lili Wang;Anping Chen;Nai-Qin Zhong;Ning Liu;Xiao-Min Wu;F. Wang;Chun-Lin Yang;M. Romero;
通讯作者：
Lili Wang;Anping Chen;Nai-Qin Zhong;Ning Liu;Xiao-Min Wu;F. Wang;Chun-Lin Yang;M. Romero;
影响因子：
7.2
作者：
Gerbeau, P;Güclü, J;Maurel, C
通讯作者：
Maurel, C
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen