Nanoscopic leg irons: harvesting of polymer-stabilized membrane proteins with antibody-functionalized silica nanoparticles.
Nanoscopic leg irons: harvesting of polymer-stabilized membrane proteins with antibody-functionalized silica nanoparticles.
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纳米级脚镣：用抗体功能化的二氧化硅纳米颗粒收获聚合物稳定的膜蛋白。
DOI：
10.1039/c5bm00133a
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发表时间：
2015
影响因子：
6.6
通讯作者：
E. Sinner
中科院分区：
文献类型：
--
作者：
T. Zapf;C. Zafiu;C. Zaba;C. Tan;W. Hunziker;E. Sinner
采用硅基纳米颗粒(SiNPs)制备复合膜蛋白，通过体外膜辅助蛋白合成(IMAP)将其包埋到单胺多聚体中。尺寸优化的SiNPs已经用聚合物靶向抗体进行了表面修饰，这些抗体被用来获得含有蛋白质的聚合体。聚合体模仿细胞膜。它们是化学定义的，并保持其结构-功能的完整性，因为几乎任何膜蛋白物种都可以通过细胞裂解物的核糖体环境合成到这种结构中。SiNPs就像“沉重的腿铁”一样捕捉聚合体，以便能够基于重力从细胞裂解物的粗略混合物中提纯和浓缩这种蛋白聚合体。
Silica-based nanoparticles (SiNPs) are presented to harvest complex membrane proteins, which have been embedded into unilammelar polymersomes via in vitro membrane assisted protein synthesis (iMAP). Size-optimized SiNPs have been surface-modified with polymer-targeting antibodies, which are employed to harvest the protein-containing polymersomes. The polymersomes mimic the cellular membrane. They are chemically defined and preserve their structural-functional integrity as virtually any membrane protein species can be synthesized into such architecture via the ribosomal context of a cellular lysate. The SiNPs resemble 'heavy leg irons' catching the polymersomes in order to enable gravity-based generic purification and concentration of such proteopolymersomes from the crude mixture of cellular lysates.
影响因子：
56.9
作者：
Discher, BM;Won, YY;Hammer, DA
通讯作者：
Hammer, DA
影响因子：
1.6
作者：
Goren MA;Fox BG
通讯作者：
Fox BG