Insertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells.
Insertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells.
复制标题
插入诱变作为鉴定参与造血干细胞自我更新的基因的途径。
DOI：
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发表时间：
2000
影响因子：
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通讯作者：
E. Spooncer
中科院分区：
文献类型：
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作者：
U. Just;D. Boettiger;O. Kan;T. Dexter;E. Spooncer
在造血系统中控制自我更新的基因仍然是未知的。使用逆转录病毒插入突变，我们已经建立了多能造血干细胞系（FDCP混合物），具有增加的自我更新能力在体外。为了鉴定参与自我更新调节的基因，从FDCP-混合物细胞克隆前病毒整合位点，并用作探针以筛选独立分离的FDCP-混合物细胞系的共同前病毒插入位点。到目前为止，已经确定了两个常见的整合位点，A25和M4。A25在50%的FDCP混合细胞系中重排，M4在10%中重排。位于这些位点或其附近的基因可能是控制造血干细胞自我更新的候选基因。
The genes controlling self renewal in the haemopoietic system are still unknown. Using retroviral insertional mutagenesis we have established multipotent haemopoietic stem cell lines (FDCP-mix) that possess an increased self renewal capacity in vitro. To identify genes involved in the regulation of self renewal, proviral integration sites were cloned from FDCP-mix cells and used as probes to screen independently isolated FDCP-mix cell lines for a common proviral insertion site. So far, two common integration sites have been identified, A25 and M4. A25 is rearranged in 50% of the FDCP-mix cell lines and M4 in 10%. Genes located at or near these sites are likely candidates for the control of self renewal of haemopoietic stem cells.
使用逆转录病毒对小鼠种系进行诱变。
影响因子：
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作者：
Lock,LF;Jenkins,NA;Copeland,NG
通讯作者：
Copeland,NG