Genotype, development and tissue-derived variation of cell-wall properties in the lignocellulosic energy crop Miscanthus.
Genotype, development and tissue-derived variation of cell-wall properties in the lignocellulosic energy crop Miscanthus.
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DOI：
10.1093/aob/mcu054
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发表时间：
2014-10
期刊：
影响因子：
4.2
通讯作者：
Bosch M
中科院分区：
文献类型：
--
作者：
da Costa RM;Lee SJ;Allison GG;Hazen SP;Winters A;Bosch M
芒草属的物种和杂交种包含的属性使它们在目前选择的专用生物能源作物中处于领先地位。植物生物质转化为生物燃料和生物材料的一个关键性状是细胞壁质量;然而，芒属物种的细胞壁组成和生物学知识有限。本研究提供了细胞壁成分变化的数据，作为选定基因型的发育和组织类型的函数，并考虑了芒草作为可持续和可再生生物能源原料的发展的影响。细胞壁生物量分析了25个基因型，考虑不同的发展阶段和茎与叶组成的变异性，傅立叶变换中红外光谱和木质素测定。此外，使用Clostridium phytofermentans生物测定来评估细胞壁消化率和向乙醇的转化。芒草茎和叶样品之间的重要细胞壁成分的差异被发现主要与结构性碳水化合物。木质素含量随着植株成熟而增加，茎组织中木质素含量较高。虽然茎木质素浓度与乙醇产量呈负相关，没有观察到这种相关性的叶子。叶组织在所有阶段对地上总生物量的贡献都很大，尽管这种贡献的程度是依赖于基因型的。据推测，不同的碳水化合物组成和修改茎和叶组织中观察到的细胞壁质量的差异是主要的决定因素。研究结果表明，木质纤维原料的改进应包括组织依赖性的变化，因为它影响生物转化的顺应性。对于与细胞壁质量相关的基因-性状关联，数据支持叶和茎组成的单独检查，因为组织特异性性状可能仅通过考虑总地上生物量样品来掩盖，并且样品变异性可能主要是由于不同组织对总生物量的贡献。
Species and hybrids of the genus Miscanthus contain attributes that make them front-runners among current selections of dedicated bioenergy crops. A key trait for plant biomass conversion to biofuels and biomaterials is cell-wall quality; however, knowledge of cell-wall composition and biology in Miscanthus species is limited. This study presents data on cell-wall compositional changes as a function of development and tissue type across selected genotypes, and considers implications for the development of miscanthus as a sustainable and renewable bioenergy feedstock. Cell-wall biomass was analysed for 25 genotypes, considering different developmental stages and stem vs. leaf compositional variability, by Fourier transform mid-infrared spectroscopy and lignin determination. In addition, a Clostridium phytofermentans bioassay was used to assess cell-wall digestibility and conversion to ethanol. Important cell-wall compositional differences between miscanthus stem and leaf samples were found to be predominantly associated with structural carbohydrates. Lignin content increased as plants matured and was higher in stem tissues. Although stem lignin concentration correlated inversely with ethanol production, no such correlation was observed for leaves. Leaf tissue contributed significantly to total above-ground biomass at all stages, although the extent of this contribution was genotype-dependent. It is hypothesized that divergent carbohydrate compositions and modifications in stem and leaf tissues are major determinants for observed differences in cell-wall quality. The findings indicate that improvement of lignocellulosic feedstocks should encompass tissue-dependent variation as it affects amenability to biological conversion. For gene–trait associations relating to cell-wall quality, the data support the separate examination of leaf and stem composition, as tissue-specific traits may be masked by considering only total above-ground biomass samples, and sample variability could be mostly due to varying tissue contributions to total biomass.
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