A programmable microenvironment for cellular studies via microfluidics-generated double emulsions.
A programmable microenvironment for cellular studies via microfluidics-generated double emulsions.
复制标题
通过微流体生成的双重乳液进行细胞研究的可编程微环境。
DOI：
10.1016/j.biomaterials.2013.03.002
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发表时间：
2013-06
期刊：
影响因子：
14
通讯作者：
Leong, Kam W.
中科院分区：
文献类型：
--
作者：
Zhang, Ying;Ho, Yi-Ping;Chiu, Ya-Ling;Chan, Hon Fai;Chlebina, Ben;Schuhmann, Tom;You, Lingchong;Leong, Kam W.
高通量细胞研究需要小的样品体积以降低成本并提高灵敏度。特别地，微流体产生的油包水（W/O）单乳液液滴系统为细胞培养、筛选和分选提供均匀、明确限定和离散的微环境。然而，这些单一乳液液滴不能连续供应营养分子，并且与基于水相的分析不相容。一种解决方案是将W/O液滴截留在另一水相中，形成水包油包水（W/O/W）双重乳液。外部水相有效地防止了干燥并减少了有机组分的量，但仍保留了区室化的优点。内部环境也可以动态编程，而不需要使液滴破裂。在这项研究中，我们探讨了潜在的应用W/O/W复乳液滴的细胞培养，基因激活和更复杂的生物事件，如细菌群体感应为例的研究。该研究表明了复乳法在复杂生物过程研究中的优势和潜在应用。
High throughput cellular studies require small sample volume to reduce costs and enhance sensitivity. Microfluidics-generated water-in-oil (W/O) single emulsion droplet systems, in particular, provide uniform, well defined and discrete microenvironment for cell culture, screening, and sorting. However, these single emulsion droplets are incapable of continuous supply of nutrient molecule and are not compatible with aqueous phase-based analysis. A solution is to entrap W/O droplets in another aqueous phase, forming water-in-oil-in-water (W/O/W) double emulsions. The external aqueous phase efficiently prevents desiccation and reduces the amount of organic component, and yet retaining the advantages of compartmentalization. The internal environment can also be programmed dynamically without the need of rupturing the droplets. In this study, we explore the potential application of W/O/W double emulsion droplets for cell cultivation, genetic activation and study of more complicated biological events such as bacteria quorum-sensing as an example. This study demonstrates the advantages and potential application of double emulsion for the study of complex biological processes.
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影响因子：
6.1
作者：
Burns, JR;Ramshaw, C
通讯作者：
Ramshaw, C
影响因子：
3.9
作者：
Barbier, Valessa;Tatoulian, Michael;Tabeling, Patrick
通讯作者：
Tabeling, Patrick
影响因子：
6.1
作者：
Baret, Jean-Christophe;Miller, Oliver J.;Griffiths, Andrew D.
通讯作者：
Griffiths, Andrew D.
影响因子：
4.1
作者：
Wang, Ai-Jun;Xu, Jing-Juan;Chen, Hong-Yuan
通讯作者：
Chen, Hong-Yuan
DOI：
10.1088/0960-1317/21/5/054032
发表时间：
2011-05-01
影响因子：
2.3
作者：
Wu, N.;Oakeshott, J. G.;Zhu, Y.
通讯作者：
Zhu, Y.