Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway.
Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway.
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淫羊藿苷通过经典Wnt信号通路调节骨髓间充质干细胞的双向分化
DOI：
10.1155/2017/8085325
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Guo FJ
中科院分区：
文献类型：
--
作者：
Huang JM;Bao Y;Xiang W;Jing XZ;Guo JC;Yao XD;Wang R;Guo FJ
在一些绝经后妇女中很容易观察到骨髓内的脂肪浸润。这些脂肪主要来源于骨髓间充质干细胞（BMMSCs）。骨髓间充质干细胞来源的脂肪细胞增多导致骨髓间充质干细胞来源的成骨细胞减少，因此骨髓间充质干细胞的双向分化在骨质疏松症中起重要作用。淫羊藿苷是中药淫羊藿的主要提取物，在中药中有着广泛的应用。本实验通过实时荧光定量PCR、免疫荧光、western blot和组织切片等方法，研究了淫羊藿苷对体外和体内BMMSCs双向分化的影响。通过染色和基因表达检测发现，淫羊藿苷对成骨有明显的促进作用，对成脂有明显的抑制作用。显微CT分析显示，淫羊藿苷治疗减轻了卵巢切除（OVX）小鼠股骨远端松质骨的丢失。H&E染色分析显示，淫羊藿苷治疗的OVX小鼠获得了更高的骨量和更少的骨髓脂滴比OVX小鼠。Western blot和免疫荧光结果显示，淫羊藿苷通过经典的Wnt信号通路调控BMMSCs的双向分化。本研究表明淫羊藿苷通过经典的Wnt信号通路调节BMMSCs的双向分化而发挥其抗肿瘤作用。
Fat infiltration within the bone marrow is easily observed in some postmenopausal women. Those fats are mainly derived from bone marrow mesenchymal stem cells (BMMSCs). The increment of adipocytes derived from BMMSCs leads to decreased osteoblasts derived from BMMSCs, so the bidirectional differentiation of BMMSCs significantly contributes to osteoporosis. Icariin is the main extractive of Herba Epimedii which is widely used in traditional Chinese medicine. In this experiment, we investigated the effect of icariin on the bidirectional differentiation of BMMSCs through quantitative real-time PCR, immunofluorescence, western blot, and tissue sections in vitro and in vivo. We found that icariin obviously promotes osteogenesis and inhibits adipogenesis through detecting staining and gene expression. Micro-CT analysis showed that icariin treatment alleviated the loss of cancellous bone of the distal femur in ovariectomized (OVX) mice. H&E staining analysis showed that icariin-treated OVX mice obtained higher bone mass and fewer bone marrow lipid droplets than OVX mice. Western blot and immunofluorescence showed that icariin regulates the bidirectional differentiation of BMMSCs via canonical Wnt signaling. This study demonstrates that icariin exerts its antiosteoporotic effect by regulating the bidirectional differentiation of BMMSCs through the canonical Wnt signaling pathway.
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