Analysis of citrate in low-volume seminal fluid samples using a time-gated measurement of europium luminescence.
Analysis of citrate in low-volume seminal fluid samples using a time-gated measurement of europium luminescence.
复制标题
使用铕发光的时间选通测量分析少量精液样品中的柠檬酸盐。
DOI：
10.1016/j.jpba.2011.05.023
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发表时间：
2011
影响因子：
3.4
通讯作者：
Pal R
中科院分区：
文献类型：
--
作者：
Pal R
这项工作的目的是开发和验证一种快速的分析方法，使精液样本中的柠檬酸盐的测量。样本来自输精管切除术后9周内的男性。两个年龄范围进行了检查，40-43岁和50-53岁之间，每种情况下有近100个样本。这项匿名研究没有患者临床病史，模拟了随机筛选队列。采用铕发射发光法测定0.5 μL精液样品中柠檬酸盐的浓度。这涉及两个分离良好的铕（III）发射带的比率分析。光谱数据获得使用的时间选通光谱仪的构造和修改进行了说明。柠檬酸盐值通过使用柠檬酸裂解酶酶促测定的独立测量和通过精液的700 MHz 1H NMR分析来确认。年龄组之间的柠檬酸盐浓度无统计学差异，40-43岁组的平均浓度为35.0（±14.6）mM，50-53岁队列的平均浓度为28.2（±12.7）mM;在每种情况下均观察到多峰分布。
This work aims to develop and validate a rapid analytical method that enables the measurement of citrate in seminal fluid samples. Samples were obtained from men who were within 9 weeks of a vasectomy operation. Two age ranges were examined, between 40–43 and 50–53 years old, with nearly 100 samples in each case. No patient clinical history was available for this anonymous study, simulating a random screening cohort. The concentration of citrate in 0.5 μL seminal fluid samples was assessed, using a europium emission luminescence method. This involves the ratiometric analysis of two well-separated europium(III) emission bands. Spectral data were obtained using a time-gated spectrometer whose construction and modification is described. Citrate values were confirmed by independent measurements using a citrate lyase enzymatic assay and by 700 MHz1H NMR analysis of the seminal fluid. Citrate concentrations were not statistically different between age groups and averaged 35.0(±14.6) mM for the 40–43 group, and 28.2(±12.7) mM for the 50–53 cohort; in each case a polymodal distribution was observed.
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影响因子：
4.8
作者：
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通讯作者：
Franklin, R. B.
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影响因子：
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DOI：
10.1016/s0022-5347(17)70403-x
发表时间：
1944
期刊：
The Journal of Urology
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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P. Tiwari;M. Rosen;A. Madabhushi