Cloning, sequence, and expression of a lipase gene from Pseudomonas cepacia: lipase production in heterologous hosts requires two Pseudomonas genes
Cloning, sequence, and expression of a lipase gene from Pseudomonas cepacia: lipase production in heterologous hosts requires two Pseudomonas genes
复制标题
洋葱假单胞菌脂肪酶基因的克隆、序列和表达：异源宿主中脂肪酶的生产需要两个假单胞菌基因
DOI：
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发表时间：
1991
影响因子：
3.2
通讯作者：
B. Diderichsen
中科院分区：
文献类型：
--
作者：
S. Jørgensen;K. W. Skov;B. Diderichsen
克隆并测序了洋葱假单胞菌胞外脂肪酶基因。在脂肪酶基因的下游鉴定出一个开放阅读框，并将其命名为limA。lipA仅在limA存在时表达良好。limA在顺式和反式中都发挥作用，因此产生可扩散的基因产物，可能是344个氨基酸的蛋白质。在大肠杆菌、枯草芽孢杆菌和lividans链霉菌中，来自革兰氏阳性菌的异源信号替代了lipA表达信号（启动子、核糖体结合位点和信号肽编码序列），仍然导致lipA依赖性的lipA表达。
The lipA gene encoding an extracellular lipase from Pseudomonas cepacia was cloned and sequenced. Downstream from the lipase gene an open reading frame was identified, and the corresponding gene was named limA. lipA was well expressed only in the presence of limA. limA exerts its effect both in cis and in trans and therefore produces a diffusible gene product, presumably a protein of 344 amino acids. Replacement of the lipA expression signals (promoter, ribosome-binding site, and signal peptide-coding sequences) by heterologous signals from gram-positive bacteria still resulted in limA-dependent lipA expression in Escherichia coli, Bacillus subtilis, and Streptomyces lividans.
影响因子：
14.9
作者：
WEST, SEH;IGLEWSKI, BH
通讯作者：
IGLEWSKI, BH
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S