Identifying the inertial cavitation threshold and skull effects in a vessel phantom using focused ultrasound and microbubbles.
Identifying the inertial cavitation threshold and skull effects in a vessel phantom using focused ultrasound and microbubbles.
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DOI：
10.1016/j.ultrasmedbio.2010.02.009
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发表时间：
2010-05
影响因子：
2.9
通讯作者：
Konofagou, Elisa E.
中科院分区：
文献类型：
--
作者：
Tung, Yao-Sheng;Choi, James J.;Baseri, Babak;Konofagou, Elisa E.
聚焦超声（FUS）结合微泡已被证明能够通过打开血脑屏障（BBB）将大分子输送到脑实质。然而，开放背后的机制仍然未知。为了研究超声过程中预制微泡惯性空化的压力阈值，采用了结合b模成像的被动空化检测方法。通过在聚丙烯酰胺凝胶中产生直径为610微米的圆柱形孔并使其体积充满微泡来模拟脑血管。在超声（频率：1.525 MHz，脉冲长度：100周期，PRF: 10 Hz，超声持续时间：2 s）之前，通过切除的小鼠颅骨注射明确微泡（平均直径范围：1.1-3.3µm, Lantheus Medical Imaging， N. Billerica， MA， USA）。利用圆柱聚焦水听器、与FUS换能器共聚焦以及视场垂直于FUS波束的线性阵列换能器被动检测空化响应引起的声发射。被动空化探测器（PCD）的宽带光谱响应和b模图像分别识别了惯性空化的发生和位置。结果表明，无论颅骨是否存在，峰值压力阈值约为0.45 MPa。小鼠颅骨不影响惯性空化阈值，但导致惯性空化剂量降低。宽带响应可以通过小鼠颅骨捕获，因此相同的PCD设置可以用于未来的体内应用。
Focused ultrasound (FUS) in combination with microbubbles has been shown capable of delivering large molecules to the brain parenchyma through opening of the blood-brain barrier (BBB). However, the mechanism behind the opening remains unknown. To investigate the pressure threshold for inertial cavitation of preformed microbubbles during sonication, passive cavitation detection in conjunction with B-mode imaging was used. A cerebral vessel was simulated by generating a cylindrical hole of 610 µm in diameter inside a polyacrylamide gel and saturating its volume with microbubbles. Definity microbubbles (Mean diameter range: 1.1–3.3 µm, Lantheus Medical Imaging, N. Billerica, MA, USA) were injected prior to sonication (frequency: 1.525 MHz; pulse length: 100 cycles; PRF: 10 Hz; sonication duration: 2 s) through an excised mouse skull. The acoustic emissions due to the cavitation response were passively detected using a cylindrically focused hydrophone, confocal with the FUS transducer and a linear-array transducer with the field of view perpendicular to the FUS beam. The broadband spectral response acquired at the passive cavitation detector (PCD) and the B-mode images identified the occurrence and location of the inertial cavitation, respectively. Findings indicated that the peak-rarefactional pressure threshold was approximately equal to 0.45 MPa, with or without the skull present. Mouse skulls did not affect the threshold of inertial cavitation but resulted in a lower inertial cavitation dose. The broadband response could be captured through the murine skull, so the same PCD set-up can be used in future in vivo applications.
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影响因子：
3.5
作者：
Chomas, JE;Dayton, P;Ferrara, K
通讯作者：
Ferrara, K
影响因子：
19.7
作者：
Hynynen, K;McDannold, N;Jolesz, FA
通讯作者：
Jolesz, FA
影响因子：
2.9
作者：
Choi, James J.;Wang, Shougang;Tung, Yao-Sheng;Morrison, Barclay, III;Konofagou, Elisa E.
通讯作者：
Konofagou, Elisa E.
DOI：
10.1109/tbme.2009.2034533
发表时间：
2010-01
期刊：
IEEE transactions on bio-medical engineering
影响因子：
--
作者：
Choi JJ;Feshitan JA;Baseri B;Wang S;Tung YS;Borden MA;Konofagou EE
通讯作者：
Konofagou EE
影响因子：
2.9
作者：
Takegami, K;Kaneko, Y;Nagawa, H
通讯作者：
Nagawa, H